Comparison of the effects of host-associated (autochthonous) and commercial probiotics on immune responses, growth parameters and intestinal microbiota of Caspian whitefish (Rutilus frisii kutum) fry

被引：3

作者：

Hoseinifar, Seyed Hossein ^{[1
]}

Hoseini, Seyyed Morteza ^{[2
]}

Mirghaed, Ali Taheri ^{[3
]}

Ghelichpour, Melika ^{[2
]}

Shirzad-Aski, Hesamaddin ^{[4
]}

Van Doan, Hien ^{[5
,6
]}

El-Haroun, Ehab ^{[7
]}

Safari, Roghieh ^{[1
]}

Khanzadeh, Majid ^{[1
,8
]}

机构：

[1] Gorgan Univ Agr Sci & Nat Resources, Fac Fisheries & Environm Sci, Dept Fisheries, Gorgan, Iran

[2] Agr Res Educ & Extens Org, Iranian Fisheries Sci Res Inst, Inland Waters Aquat Resources Res Ctr, Gorgan, Iran

[3] Univ Tehran, Fac Vet Med, Dept Aquat Anim Hlth, Tehran, Iran

[4] Golestan Univ Med Sci, Infect Dis Res Ctr, Gorgan, Iran

[5] Chiang Mai Univ, Fac Agr, Dept Anim & Aquat Sci, Chiang Mai, Thailand

[6] Chiang Mai Univ, Fac Agr, Funct Feed Innovat Ctr FuncFeed, Chiang Mai, Thailand

[7] Cairo Univ, Fish Nutr Res Lab, Anim Prod Dept, Fac Agr, Cairo, Egypt

[8] Tehran Org, Acad Ctr Educ Culture & Res ACECR, Anim Biol Prod Res Grp, Tehran, Iran

来源：

FRONTIERS IN MARINE SCIENCE | 2024年 / 11卷

关键词：

whitefish; autochthonous probiotic; Pediococcus pentosaceus; growth indicators; nonspecific immunity; TROUT ONCORHYNCHUS-MYKISS; RAINBOW-TROUT; STRESS RESISTANCE; SKIN MUCUS; PEDIOCOCCUS-ACIDILACTICI; LACTOBACILLUS-PLANTARUM; AEROMONAS-HYDROPHILA; DISEASE RESISTANCE; PERFORMANCE; AQUACULTURE;

D O I：

10.3389/fmars.2024.1446927

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Probiotics are helpful bacteria that safeguard host animals from harmful pathogens. In fish farming, the primary aim of using probiotics is to preserve or reestablish a balance between pathogenic microorganisms and the native bacteria that constitute the intestinal and skin microbial communities of fish. This study investigates the effects of host-associated probiotic (HAP) vs commercial probiotic (CP) on the growth performance, antioxidant defense and immunity of Caspian whitefish fry. Three hundred whitefish fry (1.15 +/- 0.03 g) were randomly divided into five treatments in triplicate. Treatments included feeding with the control group (zero), P. acidilactici as a commercial probiotic (CP) at 6 x 10(8) CFU g(-1), and HA Pediococcus pentosaceus at 10(6), 10(7) and 10(8) CFU g(-1) for eight weeks. Dietary HAP and CP did not have significant effects on growth indices compared to the control group (P > 0.05). However, HAP at 10(7) and 10(8) CFU g(-1) and CP significantly increased protein in whitefish carcasses compared to the control group (P < 0.05). Different levels of HAP and CP had a significant effect on whole-body extract (WBE) lysozyme (LZM) activity (P < 0.05). HAP treatment significantly increased WBE ACH50 and bactericidal activity compared to the control and CP group (P < 0.05). Also, in the case of mucosal immune response, different levels of HAP could significantly increase LZM, total immunoglobulin (Ig), agglutination titer, protease and alkaline phosphatase activity compared to the control group (P < 0.05). Whitefish fed HAP showed a significant increase in the activity of WBE antioxidant parameters (SOD, CAT and GPx) compared to the control group (P < 0.05). Also, feeding with HAP could significantly increase autochthonous LAB levels compared control group (P < 0.05); while the total count of intestinal heterotrophic bacteria was not affected (P > 0.05). Overall, the present study showed HA Pediococcus pentosaceus can be considered as beneficial feed additive for whitefish.

引用

页数：16

共 74 条

[1] A multispecies probiotic modulates growth, digestive enzymes, immunity, hepatic antioxidant activity, and disease resistance of Pangasianodon hypophthalmus fingerlings [J].