Sclerostin Antibody-Loaded Dense Collagen Hydrogels Promote Critical-Size Bone Defect Repair

被引:1
作者
Sicard, Ludovic [1 ,2 ,3 ,4 ]
Maillard, Sophie [1 ,2 ,3 ]
Akoa, Daline Mbita [5 ]
Torrens, Coralie [1 ,2 ,3 ]
Collignon, Anne-Margaux [1 ,2 ,3 ,4 ]
Coradin, Thibaud [5 ]
Chaussain, Catherine [1 ,2 ,3 ,4 ,6 ]
机构
[1] Univ Paris Cite, Inst Malad Musculo Squelett, Orofacial Pathol Imaging & Biotherapies Lab URP249, F-92120 Montrouge, France
[2] Dent Sch, FHU DDS Net, F-92120 Montrouge, France
[3] Plateforme Imagerie Vivant PIV, F-92120 Montrouge, France
[4] Univ Paris Cite, Bretonneau & Louis Mourier Hosp, AP HP, Dent Med Dept, F-75018 Paris, France
[5] Sorbonne Univ, Lab Chim Matiere Condensee Paris LCMCP, UMR 7574, CNRS, F-75005 Paris, France
[6] Univ Paris Cite, Bretonneau Hosp,AP HP,GHN, Reference Ctr Rare Disorders Calcium & Phosphate M, Dent Med Dept, F-75018 Paris, France
关键词
bone tissue engineering; monoclonal antibody therapy; sclerostin; collagen hydrogels; dental pulpstem cells; PULP STEM-CELLS; DRUG-DELIVERY; PLASTIC COMPRESSION; REGENERATION; SCAFFOLDS; BIOMATERIALS; SYSTEMS; DISEASE; GROWTH;
D O I
10.1021/acsbiomaterials.4c00883
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
The management of extensive bone loss remains a clinical challenge. Numerous studies are underway to develop a combination of biomaterials, biomolecules, and stem cells to address this challenge. In particular, the systemic administration of antibodies against sclerostin, a regulator of bone formation, was recently shown to enhance the bone repair efficiency of dense collagen hydrogels (DCHs) hosting murine dental pulp stem cells (mDPSCs). The aim of the present study was to assess whether these antibodies, encapsulated and released from DCHs, could promote craniofacial bone repair by the local inhibition of sclerostin. In vitro studies showed that antibody loading modified neither the hydrogel structure nor the viability of seeded mDPSCs. When implanted in a mouse calvaria critical-size bone defect, antibody-loaded DCHs showed repair capabilities similar to those of acellular unloaded DCHs combined with antibody injections. Importantly, the addition of mDPSCs provided no further benefit. Altogether, the local delivery of antisclerostin antibodies from acellular dense collagen scaffolds is highly effective for bone repair. The drastic reduction in the required amount of antibody compared to systemic injection should reduce the cost of the procedure, making the strategy proposed here a promising therapeutic approach for large bone defect repair.
引用
收藏
页码:6451 / 6464
页数:14
相关论文
共 84 条
[51]   Combining sclerostin neutralization with tissue engineering: An improved strategy for craniofacial bone repair [J].
Maillard, Sophie ;
Sicard, Ludovic ;
Andrique, Caroline ;
Torrens, Coralie ;
Lesieur, Julie ;
Baroukh, Brigitte ;
Coradin, Thibaud ;
Poliard, Anne ;
Slimani, Lotfi ;
Chaussain, Catherine .
ACTA BIOMATERIALIA, 2022, 140 :178-189
[52]   Cellularizing hydrogel-based scaffolds to repair bone tissue: How to create a physiologically relevant micro-environment? [J].
Maisani, Mathieu ;
Pezzoli, Daniele ;
Chassande, Olivier ;
Mantovani, Diego .
JOURNAL OF TISSUE ENGINEERING, 2017, 8
[53]   Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels [J].
Mbitta Akoa, Daline ;
Sicard, Ludovic ;
Helary, Christophe ;
Torrens, Coralie ;
Baroukh, Brigitte ;
Poliard, Anne ;
Coradin, Thibaud .
GELS, 2024, 10 (02)
[54]   Single-and Multiple-Dose Randomized Studies of Blosozumab, a Monoclonal Antibody Against Sclerostin, in Healthy Postmenopausal Women [J].
McColm, Juliet ;
Hu, Leijun ;
Womack, Theresa ;
Tang, Cheng Cai ;
Chiang, Alan Y. .
JOURNAL OF BONE AND MINERAL RESEARCH, 2014, 29 (04) :935-943
[55]   Determination of the external forces applied to the mandible during various static chewing tasks [J].
Meyer, C ;
Kahn, JL ;
Boutemy, P ;
Wilk, A .
JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY, 1998, 26 (05) :331-341
[56]   On the road to smart biomaterials for bone research: definitions, concepts, advances, and outlook [J].
Montoya, Carolina ;
Du, Yu ;
Gianforcaro, Anthony L. ;
Orrego, Santiago ;
Yang, Maobin ;
Lelkes, Peter I. .
BONE RESEARCH, 2021, 9 (01)
[57]   Dental pulp stem cells for in vivo bone regeneration: A systematic review of literature [J].
Morad, Golnaz ;
Kheiri, Lida ;
Khojasteh, Arash .
ARCHIVES OF ORAL BIOLOGY, 2013, 58 (12) :1818-1827
[58]  
Murphy MP, 2017, TISSUE ENG PART C-ME, V23, P971, DOI [10.1089/ten.tec.2017.0230, 10.1089/ten.TEC.2017.0230]
[59]   Review paper: Critical Issues in Tissue Engineering: Biomaterials, Cell Sources, Angiogenesis, and Drug Delivery Systems [J].
Naderi, Hojjat ;
Matin, Maryam M. ;
Bahrami, Ahmad Reza .
JOURNAL OF BIOMATERIALS APPLICATIONS, 2011, 26 (04) :383-417
[60]   An alginate hydrogel dura mater replacement for use with intracortical electrodes [J].
Nunamaker, Elizabeth A. ;
Kipke, Daryl R. .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2010, 95B (02) :421-429