Prevalence of Selected Immune Evasion Genes and Clonal Diversity in Methicillin-Susceptible Staphylococcus aureus Isolated from Nasal Carriers and Outpatients with Cut Wound Infections

被引:1
作者
Jura, Gabriela [1 ]
Masiuk, Helena [2 ]
Pruss, Agata [3 ]
Kurzawski, Mateusz [4 ]
Sienkiewicz, Monika [5 ]
Wojciechowska-Koszko, Iwona [1 ]
Kwiatkowski, Pawel [1 ]
机构
[1] Pomeranian Med Univ, Dept Diagnost Immunol, PL-70111 Szczecin, Poland
[2] Pomeranian Med Univ, Dept Med Analyt, PL-70111 Szczecin, Poland
[3] Pomeranian Med Univ, Dept Lab Med, PL-70111 Szczecin, Poland
[4] Pomeranian Med Univ, Lab Pharmacodynam, PL-71899 Szczecin, Poland
[5] Med Univ Lodz, Dept Pharmaceut Microbiol & Microbiol Diagnost, PL-90235 Lodz, Poland
来源
ANTIBIOTICS-BASEL | 2024年 / 13卷 / 08期
关键词
Staphylococcus aureus; MSSA; outpatients; nasal carriers; wound infections; immune evasion; clonal diversity; CHEMOTAXIS INHIBITORY PROTEIN; COMPLEMENT INHIBITOR; DISEASE CONSULTATION; BINDING PROTEIN; RESISTANT; BACTEREMIA; INNATE; CLUSTER; MRSA; MORTALITY;
D O I
10.3390/antibiotics13080730
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Staphylococcus aureus, being one of the most common human pathogens, is responsible for infections in both hospital and community settings. Its virulence is attributed to its ability to evade the immune system by producing immune evasion (IE) proteins. The aim of this study was to detect the frequency of selected IE genes (spin, sbi, sea, sak, chp, scin, sep, ecb), belonging to the immune evasion cluster (IEC), and IEC types in 86 methicillin-susceptible S. aureus (MSSA) strains isolated from unrelated outpatients. In order to determine the diversity of analyzed strains, the phylogenetic relatedness was also determined. All strains were examined for the presence of IE genes using polymerase chain reaction assay. To analyze the clonal relatedness of S. aureus, pulsed-field gel electrophoresis (PFGE) was performed. All analyzed strains harbored the scn gene, followed by sbi (95.4%), ecb (91.7%), spin (89.5%), sak (83.7%), chp (67.4%), sep (67.4%) and sea (5.8%). Seventy-three (84.9%) S. aureus strains were classified into IEC types, of which, IEC type F was most commonly observed. IEC type A was not detected. PFGE results showed no association between clonal relatedness and the presence of IE genes/IEC types. In conclusion, the abundant and so diverse repertoire of genes determining invasion in analyzed strains may prove the fact that these strains are highly advanced and adapted to evade the host immune response.
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页数:15
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