miR-146a rs2910164 (G/C) variant may predict morbid obesity risk in adults

被引:0
作者
Ozsoy, Zeki [1 ]
Nursal, Ayse Feyda [2 ]
Ozsoy, Seyma [3 ]
Tekcan, Akin [4 ]
Yigit, Serbulent [5 ]
机构
[1] Med Pk Hosp, Dept Gen Surg, Tokat, Turkiye
[2] Hitit Univ, Fac Med, Dept Med Genet, Corum, Turkiye
[3] Gaziosmanpasa Univ, Fac Med, Dept Physiol, Tokat, Turkiye
[4] Amasya Univ, Fac Med, Dept Med Biol, Amasya, Turkiye
[5] Ondokuz Mayis Univ, Fac Vet, Dept Genet, Samsun, Turkiye
关键词
Morbid obesity; miR-146a; polymorphism; PCR-RFLP; TYPE-2; DIABETES-MELLITUS; BODY-MASS INDEX; EXPRESSION; POLYMORPHISMS; ASSOCIATION; MICRORNAS; SUSCEPTIBILITY; MIRNAS;
D O I
10.1080/15257770.2024.2393323
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Obesity is a common public health problem associated with serious, life-threatening complications. MicroRNAs (miRs) have modulating roles in the immune and inflammatory systems. Therefore, this study aimed to analyze the relationship between miR-146a and morbid obesity (MO) in a Turkish population. In this study, a total of 258 subjects (110 patients with MO and 148 controls) were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to analyze miR-146a rs2910164. Then, we examined the patients as males and females separately. The results of the analyses were evaluated for statistical significance. There was a significant difference in genotype and allele frequencies of miR-146a rs2910164 between patients with MO and control individuals. miR-146a rs2910164 CC genotype and C allele were shown to increase in the MO patients' group compared to the control group (p = 0.000, p = 0.000, respectively). Also, the C allele was higher in both female and male patients compared to controls (p = 0.000, p = 0.000, respectively). High differences were also observed when the patients and the controls were compared according to CC versus GG + GC and GG versus GC + CC (p = 0.000, p = 0.000, respectively). A significant difference was found between the female/male patients and the female/male controls in terms of GG + GC versus CC (p = 0.000, p = 0.000, respectively). To the best of our knowledge, this is the first study to investigate the relationship between this variant and MO in Turkey. Our results showed that miR-146a rs2910164 is a valuable biomarker that can be used to distinguish between patients with MO and the healthy population. The findings can be extended by increasing the sample sizes with diverse ethnicities.
引用
收藏
页码:653 / 663
页数:11
相关论文
共 33 条
[1]   Health Effects of Overweight and Obesity in 195 Countries over 25 Years [J].
Afshin, Ashkan ;
Forouzanfar, Mohammad H. ;
Reitsma, Marissa B. ;
Sur, Patrick ;
Estep, Kara ;
Lee, Alex ;
Marczak, Laurie ;
Mokdad, Ali H. ;
Moradi-Lakeh, Maziar ;
Naghavi, Mohsen ;
Salama, Joseph S. ;
Vos, Theo ;
Abate, Kalkidan H. ;
Abbafati, Cristiana ;
Ahmed, Muktar B. ;
Al-Aly, Ziyad ;
Alkerwi, Ala'a ;
Al-Raddadi, Rajaa ;
Amare, Azmeraw T. ;
Amberbir, Alemayehu ;
Amegah, Adeladza K. ;
Amini, Erfan ;
Amrock, Stephen M. ;
Anjana, Ranjit M. ;
Arnlov, Johan ;
Asayesh, Hamid ;
Banerjee, Amitava ;
Barac, Aleksandra ;
Baye, Estifanos ;
Bennett, Derrick A. ;
Beyene, Addisu S. ;
Biadgilign, Sibhatu ;
Biryukov, Stan ;
Bjertness, Espen ;
Boneya, Dube J. ;
Campos-Nonato, Ismael ;
Carrero, Juan J. ;
Cecilio, Pedro ;
Cercy, Kelly ;
Ciobanu, Liliana G. ;
Cornaby, Leslie ;
Damtew, Solomon A. ;
Dandona, Lalit ;
Dandona, Rakhi ;
Dharmaratne, Samath D. ;
Duncan, Bruce B. ;
Eshrati, Babak ;
Esteghamati, Alireza ;
Feigin, Valery L. ;
Fernandes, Joao C. .
NEW ENGLAND JOURNAL OF MEDICINE, 2017, 377 (01) :13-27
[2]   Expression of Circulating MicroRNAs and Myokines and Interactions with Serum Osteopontin in Type 2 Diabetic Patients with Moderate and Poor Glycemic Control: A Biochemical and Molecular Study [J].
Al-Rawaf, Hadeel A. ;
Alghadir, Ahmad H. ;
Gabr, Sami A. .
BIOMED RESEARCH INTERNATIONAL, 2021, 2021
[3]   The rs2910164 variant is associated with reduced miR-146a expression but not cytokine levels in patients with type 2 diabetes [J].
Alipoor, B. ;
Ghaedi, H. ;
Meshkani, R. ;
Omrani, M. D. ;
Sharifi, Z. ;
Golmohammadi, T. .
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION, 2018, 41 (05) :557-566
[4]   Changes in Blood microRNA Expression and Early Metabolic Responsiveness 21 Days Following Bariatric Surgery [J].
Atkin, Stephen L. ;
Ramachandran, Vimal ;
Yousri, Noha A. ;
Benurwar, Manasi ;
Simper, Steven C. ;
McKinlay, Rodrick ;
Adams, Ted D. ;
Najafi-Shoushtari, S. Hani ;
Hunt, Steven C. .
FRONTIERS IN ENDOCRINOLOGY, 2019, 9
[5]   Circulating mir-21 and mir-146a are associated with increased cytokines and CD36 in Algerian obese male participants [J].
Benbaibeche, Hassiba ;
Hichami, Aziz ;
Oudjit, Brahim ;
Haffaf, El Mahdi ;
Kacimi, Ghouti ;
Koceir, Elhadj Ahmed ;
Khan, Naim Akhtar .
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY, 2022, 128 (06) :1461-1466
[6]  
Booth HP, 2017, SSM-POPUL HLTH, V3, P172, DOI 10.1016/j.ssmph.2016.12.012
[7]  
Brettfeld C, 2017, J ENDOCRINOL METAB, V7, P77, DOI 10.14740/jem399w
[8]   Genetic variants in microRNA genes: impact on microRNA expression, function, and disease [J].
Cammaerts, Sophia ;
Strazisar, Mojca ;
De Rijk, Peter ;
Del Favero, Jurgen .
FRONTIERS IN GENETICS, 2015, 6
[9]  
Deng ZF, 2020, IRAN J PUBLIC HEALTH, V49, P830
[10]   An overview of microRNAs [J].
Hammond, Scott M. .
ADVANCED DRUG DELIVERY REVIEWS, 2015, 87 :3-14