Isolation and Characterization of Thermophilic Bacteria from a Hot Spring in the State of Hidalgo, Mexico, and Geochemical Analysis of the Thermal Water

被引:4
作者
Ortega-Villar, Rosangel [1 ]
Escalante, Adelfo [2 ]
Astudillo-Melgar, Fernando [2 ]
Lizarraga-Mendiola, Liliana [3 ]
Vazquez-Rodriguez, Gabriela A. [1 ]
Hidalgo-Lara, Maria Eugenia [4 ]
Coronel-Olivares, Claudia [1 ]
机构
[1] Univ Autonoma Estado Hidalgo, Area Academ Quim, Carretera Pachuca Tulancingo Km 4-5, Hidalgo 42184, Mexico
[2] Univ Nacl Autonoma Mexico, Dept Ingn Celular & Biocatalisis, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
[3] Univ Autonoma Estado Hidalgo, Area Acad Ingn & Arquitectura, Carretera Pachuca Tulancingo Km 4-5, Hidalgo 42184, Mexico
[4] CINVESTAV, Dept Biotecnol & Bioingn, Mexico City 2508, Mexico
关键词
thermal water; hydro-geochemistry; enzymes; thermophilic bacteria; firmicutes; deinococcus-thermus; PURIFICATION;
D O I
10.3390/microorganisms12061066
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hot springs worldwide can be a source of extremophilic microorganisms of biotechnological interest. In this study, samplings of a hot spring in Hidalgo, Mexico, were conducted to isolate, identify, and characterize morphologically, biochemically, and molecularly those bacterial strains with potential industrial applications. In addition, a physicochemical and geochemical examination of the hot spring was conducted to fully understand the study region and its potential connection to the strains discovered. The hot spring was classified as sulfate-calcic according to the Piper Diagram; the hydrogeochemical analysis showed the possible interactions between minerals and water. Eighteen bacterial strains were isolated with optimal growth temperatures from 50 to 55 degrees C. All strains are Gram-positive, the majority having a rod shape, and one a round shape, and 17 produce endospores. Hydrolysis tests on cellulose, pectin, and xylan agar plates demonstrated enzymatic activity in some of the strains. Molecular identification through the 16S rDNA gene allowed classification of 17 strains within the Phylum Firmicutes and one within Deinococcus-Thermus. The bacterial strains were associated with the genera Anoxybacillus, Bacillus, Anerunibacillus, Paenibacillus, and Deinococcus, indicating a diversity of bacterial strains with potential industrial applications.
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页数:22
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