A a C-protein-based indirect enzyme-linked immunosorbent assay for clinical detection of antiavian reovirus antibodies

被引：0
作者：
Yang, Xia [1 ,2 ,3 ]
Gao, Hui [1 ,2 ,3 ]
Cheng, Zhi [1 ,2 ,3 ]
Zhang, Su [1 ,2 ,3 ]
Zhao, Yimeng [1 ,2 ,3 ]
Zheng, Hao [1 ,2 ,3 ]
Gao, Li [1 ,2 ,3 ]
Cao, Hong [1 ,2 ,3 ]
Li, Xiaoqi [1 ,2 ,3 ]
Zheng, Shijun J. [1 ,2 ,3 ]
Wang, Yongqiang [1 ,2 ,3 ]
机构：
[1] Natl Key Lab Vet Publ Hlth Secur, Beijing 100193, Peoples R China
[2] Minist Agr, Key Lab Anim Epidemiol, Beijing 100193, Peoples R China
[3] China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China
来源：
POULTRY SCIENCE | 2024年 / 103卷 / 11期
基金：
中国国家自然科学基金;
关键词：
Avian reovirus; aC protein; baculovirus; eukaryotic expression; indirect ELISA; AVIAN REOVIRUS; SIGMA-B; EXPRESSION; IDENTIFICATION; DIVERSITY; ELISA; GENE;
D O I：
10.1016/j.psj.2024.104188
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China indirect immunofluorescence assay (IFA; 1:3200 diluted in serum), and it showed no cross-reactivity with antibodies against other common avian viruses (such as Infectious bursal disease virus, Newcastle disease virus). The practicality of the iELISA was further evaluated using clinical samples. 300 clinical sera from chickens vaccinated with the ARV attenuated vaccine and 20 SPF sera were tested using both the iELISA and the IFA, demonstrating a 100% conformity rate. In conclusion, these results suggest that the iELISA developed in this study is a rapid, sensitive, and specific method that could serve as an effective diagnostic tool for monitoring and controlling avian viral arthritis.
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页数：8
相关论文
共 31 条
[1]   Phenotypic, genotypic and antigenic characterization of emerging avian reoviruses isolated from clinical cases of arthritis in broilers in Saskatchewan, Canada [J].
Ayalew, Lisanework E. ;
Gupta, Ashish ;
Fricke, Jenny ;
Ahmed, Khawaja Ashfaque ;
Popowich, Shelly ;
Lockerbie, Betty ;
Tikoo, Suresh K. ;
Ojkic, Davor ;
Gomis, Susantha .
SCIENTIFIC REPORTS, 2017, 7
[2]   Avian reovirus:: Structure and biology [J].
Benavente, Javier ;
Martinez-Costas, Jose .
VIRUS RESEARCH, 2007, 123 (02) :105-119
[3]   Effect of the extra N-terminal methionine residue on the stability and folding of recombinant α-lactalbumin expressed in Escherichia coli [J].
Chaudhuri, TK ;
Horii, K ;
Yoda, T ;
Arai, M ;
Nagata, S ;
Terada, TP ;
Uchiyama, H ;
Ikura, T ;
Tsumoto, K ;
Kataoka, H ;
Matsushima, M ;
Kuwajima, K ;
Kumagai, I .
JOURNAL OF MOLECULAR BIOLOGY, 1999, 285 (03) :1179-1194
[4]   Chicken infectious anemia virus (CIAV) VP1 antagonizes type I interferon (IFN-I) production by inhibiting TBK1 phosphorylation [J].
Chen, Juncheng ;
Yuan, Xu ;
Ma, Ziyue ;
Wang, Guoyan ;
Wang, Yongqiang ;
Cao, Hong ;
Li, Xiaoqi ;
Zheng, Shijun J. ;
Gao, Li .
VIRUS RESEARCH, 2023, 327
[5]   Expression of the human activin type I and II receptor extracellular domains in Pichia pastoris [J].
Daly, R ;
Hearn, MTW .
PROTEIN EXPRESSION AND PURIFICATION, 2006, 46 (02) :456-467
[6]   Emerging new avian reovirus variants from cases of enteric disorders and arthritis/tenosynovitis in Brazilian poultry flocks [J].
De la Torre, D. ;
Astolfi-Ferreira, C. S. ;
Chacon, R. D. ;
Puga, B. ;
Piantino Ferreira, A. J. .
BRITISH POULTRY SCIENCE, 2021, 62 (03) :361-372
[7]   An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis [J].
Dulwich, Katherine L. ;
Asfor, Amin S. ;
Gray, Alice G. ;
Nair, Venugopal ;
Broadbent, Andrew J. .
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2018, (140)
[8]   ISOLATION AND CHARACTERIZATION OF AN ENTEROVIRUS FROM BABY CHICKS HAVING AN ENTERIC INFECTION .2. PHYSICAL AND CHEMICAL CHARACTERISTICS AND ULTRASTRUCTURE [J].
DUTTA, SK ;
POMEROY, BS .
AVIAN DISEASES, 1967, 11 (01) :9-&
[9]  
FAHEY J. E., 1954, CANADIAN JOUR COMP MED AND VET SCI, V18, P13
[10]   The baculovirus expression vector system: A commercial manufacturing platform for viral vaccines and gene therapy vectors [J].
Felberbaum, Rachael S. .
BIOTECHNOLOGY JOURNAL, 2015, 10 (05) :702-U85
1234