Mitigation of heavy metal toxicity in pigeon pea by plant growth promoting Pseudomonas alcaliphila strain PAS1 isolated from contaminated environment

The risk of arsenic contamination is rising globally, and it has negative impacts on the physiological processes and growth of plants. Metal removal from contaminated soils can be accomplished affordably and effectively with plant growth promoting rhizobacteria (PGPR)-based microbial management. From this angle, this research evaluated the mitigation of arsenic toxicity using the bacteria isolated from contaminated site, Mettur, Salem district, South India. The newly isolated bacterial strain was screened for plant growth promotion potential and arsenic tolerance such as (100 ppm, 250 ppm, 500 ppm, 800 ppm and 1200 ppm). The metal tolerant rhizobacteria was identified using 16S rRNA gene sequence analysis as Pseudomonas alcaliphila strain PAS1 (GenBank accession number: OQ804624). Pigeon pea (Cajanus cajan) plants were used in pot culture experiments with varying concentrations of arsenic, (5 ppm, 10 ppm and 25 ppm) both with and without bacterial culture, for a period of 45 days. At the concentration of 25 ppm after the application of PAS1 enhanced the plant growth, protein and carbohydrate by 35.69%, 18.31% respectively. Interestingly, P. alcaliphila strain PAS1 significantly reduced the stress-induced elevated levels of proline, flavonoid, phenol and antioxidant enzyme in pigeon pea plants was 40%, 31.11%, 27.80% and 20.12%, respectively. Consequently, PAS1 may significantly reduce the adverse effects that arsenic causes to plant development in acidic soils, improve plant uptake of nutrients, and increase plant production. The findings of this study reveal that P. alcaliphila PAS1 is intrinsic for phytoremediation by reducing arsenic accumulation in the root and shoot.