Lipidomics by Nuclear Magnetic Resonance Spectroscopy and Liquid Chromatography-High-Resolution Mass Spectrometry in Osteosarcoma: A Pilot Study

被引：3

作者：

Pontes, Joao Guilherme de Moraes ^{[1
]}

Jadranin, Milka ^{[1
,2
]}

Assalin, Marcia Regina ^{[1
,3
]}

Escobar, Melissa Quintero ^{[1
]}

Stanisic, Danijela ^{[1
]}

Costa, Tassia Brena Barroso Carneiro ^{[1
]}

Lengert, Andre van Helvoort ^{[4
]}

Boldrini, Erica ^{[5
]}

da Silva, Sandra Regina Morini ^{[6
]}

Vidal, Daniel Onofre ^{[4
]}

Liu, Leticia Huan Bacellar ^{[1
]}

Maschietto, Mariana ^{[7
]}

Tasic, Ljubica ^{[1
]}

机构：

[1] Univ Estadual Campinas, Inst Chem, Lab Biol Chem, BR-13083970 Campinas, Brazil

[2] Univ Belgrade, Dept Chem, Inst Chem Technol & Met, Njegoseva 12, Belgrade 11000, Serbia

[3] Embrapa Environm, BR-13820000 Jaguariuna, Brazil

[4] Barretos Canc Hosp, Mol Oncol Res Ctr CPOM, BR-14784384 Barretos, Brazil

[5] Barretos Childrens Canc Hosp, BR-14784400 Barretos, Brazil

[6] Barretos Canc Hosp, Dept Pathol, BR-14784400 Barretos, Brazil

[7] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, BR-13083100 Campinas, Brazil

来源：

METABOLITES | 2024年 / 14卷 / 08期

基金：

瑞典研究理事会;

关键词：

bone tumor; osteosarcoma; metastasis; nuclear magnetic resonance spectroscopy; mass spectrometry; METABOLISM; CANCER; METASTASES;

D O I：

10.3390/metabo14080416

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cancer is a complex disease that can also affect the younger population; however, it is responsible for a relatively high mortality rate of children and youth, especially in low- and middle-income countries (LMICs). Besides that, lipidomic studies in this age range are scarce. Therefore, we analyzed blood serum samples from young patients (12 to 35 years) with bone sarcoma (osteosarcoma) and compared their lipidomics to the ones from the control group of samples, named healthy control (HC group), using NMR and LC-MS techniques. Furthermore, differences in the lipidomic profiles between OS patients with and without metastasis indicate higher glycerophosphocholine (GPC) and glycerophospholipid (GPL) levels in osteosarcoma and increased cholesterol, choline, polyunsaturated fatty acids (PUFAs), and glycerols during the metastasis. These differences, detected in the peripheral blood, could be used as biomarkers for liquid biopsy.

引用

页数：17

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