Immunogenicity and Protective Efficacy of Aerosolized Live-Attenuated Yellow Fever 17D Vaccine in Mice

被引：0
作者：
Zhu, Feng [1 ,2 ]
Sun, Meng-Xu [2 ]
Zhao, Suo-Qun [2 ]
Qin, Cheng-Feng [2 ]
Wang, Jin-Hua [1 ]
Deng, Yong-Qiang [2 ]
机构：
[1] Southwest Forestry Univ, Sch Life Sci, Kunming 650224, Peoples R China
[2] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, AMMS, Beijing 100071, Peoples R China
来源：
VACCINES | 2024年 / 12卷 / 08期
关键词：
yellow fever virus; aerosol; immunization route; immunogenicity; protective efficacy; DELIVERY;
D O I：
10.3390/vaccines12080856
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Yellow fever (YF), caused by the yellow fever virus (YFV), continually spreads and causes epidemics worldwide, posing a great threat to human health. The live-attenuated YF 17D vaccine (YF-17D) has been licensed for preventing YFV infection and administrated via the intramuscular (i.m.) route. In this study, we sought to determine the immunogenicity and protective efficacy of aerosolized YF-17D via the intratracheal (i.t.) route in mice. YF-17D stocks in liquids were successfully aerosolized into particles of 6 mu m. Further in vitro phenotype results showed the aerosolization process did not abolish the infectivity of YF-17D. Meanwhile, a single i.t. immunization with aerosolized YF-17D induced robust humoral and cellular immune responses in A129 mice, which is comparable to that received i.p. immunization. Notably, the aerosolized YF-17D also triggered specific secretory IgA (SIgA) production in bronchoalveolar lavage. Additionally, all immunized animals survived a lethal dose of YFV challenge in mice. In conclusion, our results support further development of aerosolized YF-17D in the future.
引用
收藏
页数：11
相关论文
共 34 条
[1]   The Yellow Fever Virus Vaccine Induces a Broad and Polyfunctional Human Memory CD8+ T Cell Response [J].
Akondy, Rama S. ;
Monson, Nathan D. ;
Miller, Joseph D. ;
Edupuganti, Srilatha ;
Teuwen, Dirk ;
Wu, Hong ;
Quyyumi, Farah ;
Garg, Seema ;
Altman, John D. ;
Del Rio, Carlos ;
Keyserling, Harry L. ;
Ploss, Alexander ;
Rice, Charles M. ;
Orenstein, Walter A. ;
Mulligan, Mark J. ;
Ahmed, Rafi .
JOURNAL OF IMMUNOLOGY, 2009, 183 (12) :7919-7930
[2]   Pulmonary delivery of influenza vaccine formulations in cotton rats: site of deposition plays a minor role in the protective efficacy against clinical isolate o H1N1pdm virus [J].
Bhide, Yoshita ;
Tomar, Jasmine ;
Dong, Wei ;
de Vries-Idema, Jacqueline ;
Frijlink, Henderik W. ;
Huckriede, Anke ;
Hinrichs, Wouter L. J. .
DRUG DELIVERY, 2018, 25 (01) :533-545
[3]   Yellow fever control: current epidemiology and vaccination strategies [J].
Chen, Lin H. ;
Wilson, Mary E. .
TROPICAL DISEASES TRAVEL MEDICINE AND VACCINES, 2020, 6 (01)
[4]   Spectrum of disease outcomes in mice infected with YFV-17D [J].
Erickson, Andrea K. ;
Pfeiffer, Julie K. .
JOURNAL OF GENERAL VIROLOGY, 2015, 96 :1328-1339
[5]   Intratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice [J].
Gan, Changjiao ;
Luo, Wenbo ;
Yu, Yunzhou ;
Jiao, Zhouguang ;
Li, Sha ;
Su, Duo ;
Feng, Junxia ;
Zhao, Xiaodong ;
Qiu, Yefeng ;
Hu, Lingfei ;
Zhou, Dongsheng ;
Xiong, Xiaolu ;
Wang, Jinglin ;
Yang, Huiying .
NPJ VACCINES, 2021, 6 (01)
[6]   Yellow Fever: A Reemerging Threat [J].
Gardner, Christina L. ;
Ryman, Kate D. .
CLINICS IN LABORATORY MEDICINE, 2010, 30 (01) :237-+
[7]   Yellow Fever: Origin, Epidemiology, Preventive Strategies and Future Prospects [J].
Gianchecchi, Elena ;
Cianchi, Virginia ;
Torelli, Alessandro ;
Montomoli, Emanuele .
VACCINES, 2022, 10 (03)
[8]   Review Article: Efficacy and Duration of Immunity after Yellow Fever Vaccination: Systematic Review on the Need for a Booster Every 10 Years [J].
Gotuzzo, Eduardo ;
Yactayo, Sergio ;
Cordova, Erika .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2013, 89 (03) :434-444
[9]   The Present and Future of Yellow Fever Vaccines [J].
Hansen, Clairissa A. ;
Barrett, Alan D. T. .
PHARMACEUTICALS, 2021, 14 (09)
[10]   Yellow Fever Vaccination Elicits Broad Functional CD4+ T Cell Responses That Recognize Structural and Nonstructural Proteins [J].
James, Eddie A. ;
LaFond, Rebecca E. ;
Gates, Theresa J. ;
Mai, Duy T. ;
Malhotra, Uma ;
Kwok, William W. .
JOURNAL OF VIROLOGY, 2013, 87 (23) :12794-12804
1234