Variation in Interleukin-4,-6, and-10 in Mastitis Milk: Associations with Infections, Pathogens, Somatic Cell Counts, and Oxidative Stress

被引：1

作者：

Chaisri, Wasana ^{[1
,2
]}

Intanon, Montira ^{[1
,2
]}

Saipinta, Duanghathai ^{[1
,2
]}

Srithanasuwan, Anyaphat ^{[3
]}

Pangprasit, Noppason ^{[4
]}

Jaraja, Weerin ^{[1
]}

Chuasakhonwilai, Areerat ^{[1
]}

Suriyasathaporn, Witaya ^{[1
,2
,5
]}

机构：

[1] Chiang Mai Univ, Fac Vet Med, Chiang Mai 50100, Thailand

[2] Chiang Mai Univ, Res Ctr Producing & Dev Prod & Innovat Anim Hlth &, Chiang Mai 50100, Thailand

[3] Wageningen Univ, Dept Anim Sci, NL-6700 AH Wageningen, Netherlands

[4] Walailak Univ, Akkhraratchakumari Vet Coll, Nakhon Si Thammarat 80160, Thailand

[5] Nagoya Univ, Asian Satellite Campuses Inst, Cambodia Campus, Nagoya 4648601, Japan

来源：

VETERINARY SCIENCES | 2024年 / 11卷 / 08期

关键词：

concomitant infection; interleukin; mastitis; streptococci; staphylococci; MDA; STREPTOCOCCUS-UBERIS; INFLAMMATION; SERUM; IL-4; COWS;

D O I：

10.3390/vetsci11080350

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Poor mastitis control favors intramammary infection (IMI), which always involves CNS. This study aimed to determine the relationships of IL-4, IL-6, and IL-10 in mastitis milk with concurrent infection, bacterial pathogens, SCC, and MDA, an oxidative stress marker. All mastitis quarters from five smallholder dairy farms were sampled aseptically before morning milking and again before afternoon milking for bacteriological identification using MALDI-TOF mass spectrometry. The samples with the concomitant infection between streptococci and CNS and their pairs of another sample from the quarters were selected. In addition, samples were randomly chosen to have a controlled single infection. IL-4, IL-6, and IL-10 were measured with ELISA kits. MDA was measured using HPLC, while SCC was measured using Fossomatic (TM) FC. The results from a repeated measure analysis showed that IL-4 positively correlated with SCC, while IL-6 showed a negative trend. IL-4 levels were highest in CNS infections and significantly higher than in non-infected or mixed infections (p < 0.05). The IL-6 level of the mixed bacteria was highest and showed a different trend from non-infection, and the quarter was infected with streptococcal bacteria. In conclusion, from a single infection, the streptococci and CNS quarter showed varied immune responses, including trendily higher IL-6 and IL-4.

引用

页数：8