Chromosome-level assembly reveals a putative Y-autosomal fusion in the sex determination system of the Greenland Halibut (Reinhardtius hippoglossoides)

被引:1
作者
Ferchaud, Anne-Laure [1 ]
Merot, Claire [1 ]
Normandeau, Eric [1 ]
Ragoussis, Jiannis [2 ,3 ]
Babin, Charles [1 ]
Djambazian, Haig [2 ,3 ]
Berube, Pierre [2 ,3 ]
Audet, Celine [4 ]
Treble, Margaret [5 ]
Walkusz, Wojciech [5 ]
Bernatchez, Louis [1 ]
机构
[1] Univ Laval, Inst Biol Integrat & Syst, Dept Biol, Quebec City, PQ G1V 0A6, Canada
[2] McGill Univ, McGill Genome Ctr, Montreal, PQ H3A 0G1, Canada
[3] McGill Univ, Dept Human Genet, Montreal, PQ H3A 0G1, Canada
[4] Univ Quebec Rimouski, Dept Inst Sci Mer Rimouski, Rimouski, PQ G5L 3A1, Canada
[5] Fisheries & Oceans Canada, Freshwater Inst Winnipeg, Winnipeg Dept, Arctic Aquat Res Div, Winnipeg, MB R3T 2N6, Canada
来源
G3-GENES GENOMES GENETICS | 2021年 / 12卷 / 01期
基金
加拿大自然科学与工程研究理事会;
关键词
long-reads; PacBio; optical mapping; low-coverage whole-genome sequencing; sex determination; Y-autosomal fusion; flatfish; HEAT-INDUCIBLE DEGRON; REPLICATION PROTEINS; ESSENTIAL GENES; AUXIN; DEPLETION; INACTIVATION; EFFICIENT; VERSATILE; MODULES; POINT;
D O I
暂无
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Despite the commercial importance of Greenland Halibut (Reinhardtius hippoglossoides), important gaps still persist in our knowledge of this species, including its reproductive biology and sex determination mechanism. Here, we combined single-molecule sequencing of long reads (Pacific Sciences) with chromatin conformation capture sequencing (Hi-C) data to assemble the first chromosome-level reference genome for this species. The high-quality assembly encompassed more than 598 Megabases (Mb) assigned to 1594 scaffolds (scaffold N50 = 25 Mb) with 96% of its total length distributed among 24 chromosomes. Investigation of the syntenic relationship with other economically important flatfish species revealed a high conservation of synteny blocks among members of this phylogenetic clade. Sex determination analysis revealed that similar to other teleost fishes, flatfishes also exhibit a high level of plasticity and turnover in sex determination mechanisms. A low-coverage whole-genome sequence analysis of 198 individuals revealed that Greenland Halibut possesses a male heterogametic XY system and several putative candidate genes implied in the sex determination of this species. Our study also suggests for the first time in flatfishes that a putative Y-autosomal fusion could be associated with a reduction of recombination typical of the early steps of sex chromosome evolution.
引用
收藏
页数:13
相关论文
共 59 条
  • [1] Bähler J, 1998, YEAST, V14, P943, DOI 10.1002/(SICI)1097-0061(199807)14:10<943::AID-YEA292>3.0.CO
  • [2] 2-Y
  • [3] TATA BOX MUTATIONS IN THE SCHIZOSACCHAROMYCES-POMBE NMT-1 PROMOTER AFFECT TRANSCRIPTION EFFICIENCY BUT NOT THE TRANSCRIPTION START POINT OR THIAMINE REPRESSIBILITY
    BASI, G
    SCHMID, E
    MAUNDRELL, K
    [J]. GENE, 1993, 123 (01) : 131 - 136
  • [4] Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae
    Ben-Aroya, Shay
    Coombes, Candice
    Kwok, Teresa
    O'Donnell, Kathryn A.
    Boeke, Jef D.
    Hieter, Philip
    [J]. MOLECULAR CELL, 2008, 30 (02) : 248 - 258
  • [5] Rapid regulation of protein activity in fission yeast
    Boe, Cathrine A.
    Garcia, Ignacio
    Pai, Chen-Chun
    Sharom, Jeffrey R.
    Skjolberg, Henriette C.
    Boye, Erik
    Kearsey, Stephen
    MacNeill, Stuart A.
    Tyers, Michael D.
    Grallert, Beata
    [J]. BMC CELL BIOLOGY, 2008, 9 (1)
  • [6] A dual molecular analogue tuner for dissecting protein function in mammalian cells
    Brosh, Ran
    Hrynyk, Iryna
    Shen, Jessalyn
    Waghray, Avinash
    Zheng, Ning
    Lemischka, Ihor R.
    [J]. NATURE COMMUNICATIONS, 2016, 7
  • [7] Plant hormones: a fungal point of view
    Chanclud, Emilie
    Morel, Jean-Benoit
    [J]. MOLECULAR PLANT PATHOLOGY, 2016, 17 (08) : 1289 - 1297
  • [8] Defining the physiological role of SRP in protein-targeting efficiency and specificity
    Costa, Elizabeth A.
    Subramanian, Kelly
    Nunnari, Jodi
    Weissman, Jonathan S.
    [J]. SCIENCE, 2018, 359 (6376) : 689 - 692
  • [9] Rapid regulation of nuclear proteins by rapamycin-induced translocation in fission yeast
    Ding, Lin
    Laor, Dana
    Weisman, Ronit
    Forsburg, Susan L.
    [J]. YEAST, 2014, 31 (07) : 253 - 264
  • [10] HEAT-INDUCIBLE DEGRON - A METHOD FOR CONSTRUCTING TEMPERATURE-SENSITIVE MUTANTS
    DOHMEN, RJ
    WU, PP
    VARSHAVSKY, A
    [J]. SCIENCE, 1994, 263 (5151) : 1273 - 1276