Phytochemical, spectroscopic analysis and antifungal activity on bell peppers of hydrothermal bioactive metabolites of Humulus lupulus L. extracts

被引:0
作者
Basilio-Cortes, Ulin Antobelli [1 ]
Ramirez-Rodrigues, Milena M. [2 ]
Ramirez-Rodrigues, Melissa A. [3 ]
Gonzalez-Mendoza, Daniel [1 ]
Tzintzun-Camacho, Olivia [1 ]
Duran-Hernandez, Dagoberto [1 ]
Gonzalez-Salitre, Lourdes [4 ]
机构
[1] Autonomous Univ Baja Calif, Inst Agr Sci, Acad Area Biotechnol, Mexicali, Baja CA, Mexico
[2] Calif Polytech State Univ San Luis Obispo, Dept Food Sci & Nutr, San Luis Obispo, CA USA
[3] Intema SA CV, Food Anal Lab, Puebla, Mexico
[4] Autonomous Univ State Hidalgo, Inst Basic Sci & Engn, Acad Area Chem, City Knowledge, Mineral De La Reforma, Hidalgo, Mexico
关键词
Humulus lupulus L; LC-MS/ms; FT-IR; antifungal; bell pepper; BITTER ACIDS; IMPACT; HOPS; PRENYLFLAVONOIDS; QUANTITATION; TEMPERATURE; INGREDIENT; QUALITY; BEER;
D O I
10.1080/14786419.2024.2405010
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
This study aimed to evaluate the impact of temperature on the potential extraction of bioactive compounds from aqueous hop extract samples. The main bioactive components were characterised and analysed by LC-MS/MS, FT-IR, phenolic compounds and total flavonoids. Antifungal activity was evaluated in vitro and in vivo in bell peppers. LC-MS/MS analysis demonstrated increases and decreases of bioactive compounds in both extracts depending on the extraction temperature of 25 or 65 degrees C. The bioactive compounds showed significant changes in the bands between 2786 to 3600 cm(-1) and 1022 to 1729 cm(-1) in the FT-IR spectrum. The highest antifungal activity against the microorganisms was observed in the Ekuanot(MT) extract at an extraction temperature of 65 degrees C. The in vivo test with bell peppers presented antifungal activity during five days of evaluation under normal environmental conditions without refrigeration, presenting <= 52% of the disease due to F. oxysporum and A. solani. [GRAPHCS]
引用
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页数:12
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