Phytofabrication of Silver Nanoparticles Using Pomegranate Juice Extract for Antioxidant, Antimicrobial and Cytotoxic Activities

In the current research, monowave-assisted biosynthesis of AgNPs utilising aqueous Pomegranate Juice extract (PJE) for antioxidant, antimicrobial and cytotoxic activities were evaluated. The synthetic approach used for the biosynthesis of AgNPs was simple, affordable and eco-friendly. UV-Visible, DLS, FTIR, XRD, FE-SEM and HR-TEM techniques were used to characterize the synthesized AgNPs. The DLS technique confirmed the monodispersed nature and zeta potential of -25.8 mV. FTIR technique revealed that OH functional groups of phytochemicals were crucial for the reduction and stabilization of AgNPs. FESEM and HRTEM techniques confirmed that biosynthesized AgNPs appeared spherical and triangular. XRD analysis resulted in 18.04 nm size of AgNPs. The antioxidant capacityof biosynthesized AgNPs was analysed via DPPH (IC50 35.20 mu g/mL), ABTS (IC50 37.77 mu g/mL), FRAP (4.37 mM Fe(II)/mL at 90 mu g/mL) and total antioxidant (77.16 g AAE/mL at 90 mu g/mL) assays. Biosynthesized AgNPs exhibited antifungal activity against Colletotrichum gloesporioides, Colletotrichum graminicola and antibacterial activity against Xanthomonas axonopodis, Klebsiella aerogenes at 50 mu g/mL. Owing to these studies, synthesized AgNPs may be valuable in the future for biomedical and agricultural applications. This research focuses on a sustainable and economical method for synthesizing AgNPs utilizing Punica granatum L. juice extract at room temperature and pH 7. AgNPs were characterized using UV-Vis, DLS, FTIR, FESEM, XRD and HRTEM techniques. Synthesized AgNPs exhibited great antioxidant potential assessed through DPPH, ABTS, FRAP and TAC assays. Antimicrobial potential was assessed for crop pathogens like C. gloesporioides, C. graminicola, X. axonopodis and K. aerogenes. MTT assay was performed to check the cytotoxicity of synthesized AgNPs. image