Three-Dimensional Visualization of Breast Cancer Pathology Evolution in Clinical Patient Tissues with NIR-II Imaging

被引：3

作者：

Li, Shasha ^{[1
]}

Xin, Qi ^{[1
]}

Li, Yuan ^{[2
,3
]}

Ma, Huizhen ^{[1
,2
,3
]}

Yan, Haoyue ^{[1
]}

Ao, Siyu ^{[1
]}

Li, Hongwei ^{[1
]}

Wang, Qi ^{[4
]}

Wang, Zhidong ^{[4
]}

Liu, Pengfei ^{[1
]}

Wang, Hao ^{[1
]}

Zhang, Xiao-Dong ^{[1
,2
,3
]}

机构：

[1] Tianjin Univ, Tianjin Key Lab Brain Sci & Neural Engn, Acad Med Engn & Translat Med, Tianjin 300072, Peoples R China

[2] Tianjin Univ, Sch Sci, Dept Phys, Tianjin 300350, Peoples R China

[3] Tianjin Univ, Sch Sci, Tianjin Key Lab Low Dimens Mat Phys & Preparing Te, Tianjin 300350, Peoples R China

[4] Beijing Inst Radiat Med, Dept Radiobiol, Beijing Key Lab Radiobiol, Beijing 100850, Peoples R China

来源：

NANO LETTERS | 2024年 / 24卷 / 33期

基金：

中国国家自然科学基金;

关键词：

Gold nanoclusters; Breast cancer; Moleculartyping; Three-dimensional visualization; LIGHT-SHEET MICROSCOPY; CLUSTER;

D O I：

10.1021/acs.nanolett.4c02945

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Breast cancer (BC) is the most common tumor worldwide and requires crucial molecular typing for treatment and prognosis assessment. Currently, approaches like pathological staining, immunohistochemistry (IHC), and immunofluorescence (IF) face limitations due to the low signal-to-background ratio (SBR) and high tumor heterogeneity, resulting in a high misdiagnosis rate. Fluorescent assay in the second near-infrared region (NIR-II, 1000-1700 nm) exhibits ultrahigh SBR owing to diminished scattering and tissue autofluorescence. Here, we present a NIR-II strategy for accurate BC molecular typing and three-dimensional (3D) visualization based on the atomically precise fluorescent Au24Pr1 clusters. Single-atom Pr doping results in 3.9-fold fluorescence enhancement and long-term photostability. The Au24Pr1 clusters possess high fluorescence centered at similar to 1100 nm and the SBR on pathological section diagnosis was 4 times higher than that of NIR-I imaging. This enables high spatial resolution 3D visualization of biopsy specimens, which can surmount tissue heterogeneity for clinical diagnosis of BC.

引用

页码：10337 / 10347

页数：11

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