A study on an improved laser speckle contrast blood flow imaging methodology

The laser speckle contrast imaging technique based on the dynamic light scattering theory presents a nonscanning and wide-field method for blood flow imaging. However, its accuracy in biological tissues is limited to the decreased contrast and reduced image clarity as conventional single-exposure approaches are susceptible to static scattering. In this paper, based on the adaptive window space direction contrast (awsdK) imaging method proposed by the laboratory, combined with the optimized single exposure technology, the effect of static scattering under a single exposure is reduced. The experimental results show that the method can effectively correct static scattering and eliminate the effect of system noise on speckle contrast. This method not only improves the imaging quality, but also realizes the rapid monitoring of blood flow changes by using the speckle contrast ratio measured in a single exposure, which provides an effective solution for the further development of laser speckle contrast imaging technology.