Redox Proteomics of Protein-bound Methionine Oxidation

被引:112
作者
Ghesquiere, Bart [1 ,2 ]
Jonckheere, Veronique [1 ,2 ]
Colaert, Niklaas [1 ,2 ]
Van Durme, Joost [3 ]
Timmerman, Evy [1 ,2 ]
Goethals, Marc [1 ,2 ]
Schymkowitz, Joost
Rousseau, Frederic [3 ]
Vandekerckhove, Joel [1 ,2 ]
Gevaert, Kris [1 ,2 ]
机构
[1] VIB, Dept Med Prot Res, B-9000 Ghent, Belgium
[2] Univ Ghent, Dept Biochem, B-9000 Ghent, Belgium
[3] Vrije Univ Brussel VIB, SWITCH Lab, B-1050 Brussels, Belgium
关键词
SULFOXIDE REDUCTASES; ACTIVATION; MECHANISM; PEPTIDES; PATHWAY; LIPIDS;
D O I
10.1074/mcp.M110.006866
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We here present a new method to measure the degree of protein-bound methionine sulfoxide formation at a proteome-wide scale. In human Jurkat cells that were stressed with hydrogen peroxide, over 2000 oxidation-sensitive methionines in more than 1600 different proteins were mapped and their extent of oxidation was quantified. Meta-analysis of the sequences surrounding the oxidized methionine residues revealed a high preference for neighboring polar residues. Using synthetic methionine sulfoxide containing peptides designed according to the observed sequence preferences in the oxidized Jurkat proteome, we discovered that the substrate specificity of the cellular methionine sulfoxide reductases is a major determinant for the steady-state of methionine oxidation. This was supported by a structural modeling of the MsrA catalytic center. Finally, we applied our method onto a serum proteome from a mouse sepsis model and identified 35 in vivo methionine oxidation events in 27 different proteins. Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.006866, 1-12, 2011.
引用
收藏
页数:12
相关论文
共 39 条
  • [1] BONVINI E, 1984, J CLIN INVEST, V73, P1629, DOI 10.1172/JCI111369
  • [2] Modification of protein surface hydrophobicity and methionine oxidation by oxidative systems
    Chao, CC
    Ma, YS
    Stadtman, ER
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (07) : 2969 - 2974
  • [3] Modulation of potassium channel function by methionine oxidation and reduction
    Ciorba, MA
    Heinemann, SH
    Weissbach, H
    Brot, N
    Hoshi, T
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (18) : 9932 - 9937
  • [4] Improved visualization of protein consensus sequences by iceLogo
    Colaert, Niklaas
    Helsens, Kenny
    Martens, Lennart
    Vandekerckhove, Joel
    Gevaert, Kris
    [J]. NATURE METHODS, 2009, 6 (11) : 786 - 787
  • [5] Methionine oxidation as a major cause of the functional impairment of oxidized actin
    Dalle-Donne, I
    Rossi, R
    Giustarini, D
    Gagliano, N
    Di Simplicio, P
    Colombo, R
    Milzani, A
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 2002, 32 (09) : 927 - 937
  • [6] The oxidative environment and protein damage
    Davies, MJ
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2005, 1703 (02): : 93 - 109
  • [7] DAVID: Database for annotation, visualization, and integrated discovery
    Dennis, G
    Sherman, BT
    Hosack, DA
    Yang, J
    Gao, W
    Lane, HC
    Lempicki, RA
    [J]. GENOME BIOLOGY, 2003, 4 (09)
  • [8] A dynamic pathway for calcium-independent activation of CaMKII by methionine oxidation
    Erickson, Jeffrey R.
    Joiner, Mei-ling A.
    Guan, Xiaoqun
    Kutschke, William
    Yang, Jinying
    Oddis, Carmine V.
    Bartlett, Ryan K.
    Lowe, John S.
    O'Donnell, Susan E.
    Aykin-Burns, Nukhet
    Zimmerman, Matthew C.
    Zimmerman, Kathy
    Ham, Amy-Joan L.
    Weiss, Robert M.
    Spitz, Douglas R.
    Shea, Madeline A.
    Colbran, Roger J.
    Mohler, Peter J.
    Anderson, Mark E.
    [J]. CELL, 2008, 133 (03) : 462 - 474
  • [9] Methionine sulfoxide reductases in prokaryotes
    Ezraty, B
    Aussel, L
    Barras, F
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2005, 1703 (02): : 221 - 229
  • [10] Methionine sulfoxide reductases protect Ffh from oxidative damages in Escherichia coli
    Ezraty, B
    Grimaud, R
    El Hassouni, M
    Moinier, D
    Barras, F
    [J]. EMBO JOURNAL, 2004, 23 (08) : 1868 - 1877