Pistacia chinensis Inhibits NO Production and Upregulates HO-1 Induction via PI-3K/Akt Pathway in LPS Stimulated Macrophage Cells

被引：1

作者：

Yayeh, Taddesse ^{[1
,2
]}

Hong, Mei ^{[3
]}

Jia, Qi ^{[3
]}

Lee, Young-Chul ^{[3
]}

Kim, Hyun-Jin ^{[3
]}

Hyun, Eujin ^{[3
]}

Kim, Tae-Wan ^{[1
,2
]}

Rhee, Man Hee ^{[1
,2
]}

机构：

[1] Kyungpook Natl Univ, Lab Physiol & Cell Signaling, Coll Vet Med, Taegu 702701, South Korea

[2] Kyungpook Natl Univ, Stem Cell Res Therapeut Inst, Taegu 702701, South Korea

[3] Sch Life Unigen Inc, Cheonan 330863, Chungnam, South Korea

来源：

AMERICAN JOURNAL OF CHINESE MEDICINE | 2012年 / 40卷 / 05期

关键词：

Pistacia chinensis; Nitric Oxide; Heme Oxygenase-1; Murine Macrophages; NF-KAPPA-B; HEME OXYGENASE-1; SIGNALING PATHWAYS; MOLECULAR-MECHANISMS; COX-2; EXPRESSION; OXIDATIVE STRESS; NITRIC-OXIDE; INDUCED INOS; ACTIVATION; PROTEIN;

D O I：

10.1142/S0192415X12500802

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Pistacia chinensis has been used for various purposes in China including as an understock for grafting Pistacia vera. However, little attention was given to its health promoting effects. Therefore, in this study, we investigated the effect of Pistacia chinensis methanolic extract (PCME) containing resorcinol class of phenolic lipids on pro-inflammatory mediators and heme oxygenase-1(HO-1) in lipopolysaccharide stimulated RAW264.7 cells. While PCME (2.5-10 mu g/ml) inhibited mRNA expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and interleukin (IL)-6, it up-regulated HO-1 expression. Likewise, PCME inhibited iNOS protein expression, but not COX-2, and reduced nitric oxide (NO) release. Moreover, Phosphorylated c-Jun N-terminal Kinase (JNK) was attenuated dose-dependently in PCME pre-treated RAW264.7 cells. In addition, PCME up-regulated HO-1 protein expression was diminished by pre-treatment of PI-3K inhibitor. Furthermore, nuclear factor erythroid 2 related factor 2 (Nrf 2) repressor was attenuated time-dependently during PCME treatment. Taken together, our study showed (for the first time) that PCME inhibited NO production and up-regulated HO-1 induction via PI-3K/Akt pathway, suggesting the role of Pistacia chinensis as potential sources of anti-inflammatory and antioxidant natural compounds.

引用

页码：1085 / 1097

页数：13

共 44 条

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