Simultaneous therapeutic and diagnostic applications of magnetic PLGA nanoparticles loaded with doxorubicin in rabbit

被引：1

作者：

Salmasi, Zahra ^{[1
]}

Kamali, Hossein ^{[2
]}

Rezaee, Hanieh ^{[3
]}

Nazeran, Faezeh ^{[3
]}

Jafari, Zahra ^{[3
]}

Eisvand, Frarhad ^{[3
]}

Teymouri, Manoocher ^{[4
]}

Khordad, Elnaz ^{[5
]}

Mosafer, Jafar ^{[5
,6
]}

机构：

[1] Mashhad Univ Med Sci, Sch Pharm, Dept Pharmaceut Nanotechnol, Mashhad, Iran

[2] Mashhad Univ Med Sci, Targeted Drug Delivery Res Ctr, Sch Pharm, Mashhad, Iran

[3] Mashhad Univ Med Sci, Sch Pharm, Dept Pharmaceut, Mashhad, Iran

[4] North Khorasan Univ Med Sci, Nat Prod & Med Plants Res Ctr, Bujnord, Iran

[5] Torbat Heydariyeh Univ Med Sci, Res Ctr Adv Technol Med, Torbat Heydariyeh, Iran

[6] Torbat Heydariyeh Univ Med Sci, Hlth Sci Res Ctr, POB 9516915169, Torbat Heydariyeh, Iran

来源：

DRUG DELIVERY AND TRANSLATIONAL RESEARCH | 2025年 / 15卷 / 02期

关键词：

PLGA; Doxorubicin; SPION; MRI; Theranostic; Rabbit; PEGYLATED LIPOSOMAL DOXORUBICIN; IN-VIVO EVALUATION; PHASE-III TRIAL; POLYMERIC MICELLES; TARGETED DELIVERY; VITRO; BIODISTRIBUTION; CHEMOTHERAPY; COMBINATION; CELLS;

D O I：

10.1007/s13346-024-01693-9

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

In this study, DOX (Doxorubicin) and Fe3O4 magnetic nanocrystals (SPIONs (Superparamagnetic iron oxide nanocrystals)) were encapsulated in the PLGA-PEG: poly(lactide-co-glycolide)-b-poly(ethylene glycol) nanoparticles for theranostic purposes. The final prepared formulation which is called NPs (Nanoparticles) exhibited a particle size with a mean diameter of similar to 209 nm and a sufficient saturation magnetization value of 1.65 emu/g. The NPs showed faster DOX release at pH 5.5 compared to pH 7.4. Also, the cytotoxicity effect of NPs increased compared to Free-DOX alone in C6 glioma cancer cells. For in vivo investigations, the 2.2 Kg rabbits were injected with NPs formulations via a central articular anterior vein in their ears. Furthermore, the images of rabbit organs were depicted via MR (Magnetic resonance) and fluorescent imaging techniques. A negative contrast (dark signal) was observed in T-2 (Relaxation Time) weighted MR images of IV (Intravenously)-injected rabbits with NPs compared to the control ones. The organ's florescent images of NPs-injected rabbits showed a high density of red color related to the accumulation of DOX in liver and kidney organs. These data showed that the NPs have no cytotoxicity effect on the heart. Also, the results of histopathological tests of different organs showed that the groups receiving NPs and Free-DOX were almost similar and no significant difference was seen, except for the cardiac tissue in which the pathological effects of NPs were significantly less than the Free-DOX. Additionally, pharmacokinetic studies were also conducted at the sera and whole bloods of IV-injected rabbits with NPs and Free-DOX. The pharmacokinetic parameters showed that NPs could enhance the DOX retention in the serum compared to the Free-DOX. Altogether, we aimed to produce a powerful delivery nanosystem for its potential in dual therapeutic and diagnostic applications which are called theranostic agents.

引用

页码：770 / 785

页数：16

共 53 条

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