Harnessing the Energy Potential and Value-Added Products from the Treatment of Sugarcane Vinasse: Maximizing Methane Production Through Co-Digestion with Sugarcane Molasses and Enhanced Organic Loading

This study assessed the impact of organic loading rate (OLR) on methane (CH4) production in the anaerobic co-digestion (AcoD) of sugarcane vinasse and molasses (SVM) (1:1 ratio) within a thermophilic fluidized bed reactor (AFBR). The OLR ranged from 5 to 27.5 kg COD.m(-3).d(-1), with a fixed hydraulic retention time (HRT) of 24 h. Organic matter removal varied from 56 to 84%, peaking at an OLR of 5 kg COD.m(-3).d(-1). Maximum CH4 yield (MY) (272.6 mL CH4.g(-1)COD(rem)) occurred at an OLR of 7.5 kg COD.m(-3).d(-1), while the highest CH4 production rate (MPR) (4.0 L CH4.L-1.d(-1)) and energy potential (E.P.) (250.5 kJ.d(-1)) were observed at an OLR of 20 kg COD.m(-3).d(-1). The AFBR exhibited stability across all OLR. At 22.5 kg COD.m(-3).d(-1), a decrease in MY indicated methanogenesis imbalance and inhibitory organic compound accumulation. OLR influenced microbial populations, with Firmicutes and Thermotogota constituting 43.9% at 7.5 kg COD.m(-3).d(-1), and Firmicutes dominating (52.7%) at 27.5 kg COD.m(-3).d(-1). Methanosarcina (38.9%) and hydrogenotrophic Methanothermobacter (37.6%) were the prevalent archaea at 7.5 kg COD.m(-3).d(-1) and 27.5 kg COD.m(-3).d(-1), respectively. Therefore, this study demonstrates that the organic loading rate significantly influences the efficiency of methane production and the stability of microbial communities during the anaerobic co-digestion of sugarcane vinasse and molasses, indicating that optimized conditions can maximize energy yield and maintain methanogenic balance.