Simultaneous Identification of Vitamins B1, B3, B5, and B6 by an Engineered Nanopore

被引：2

作者：

Ouyang, Yusheng ^{[1
,2
]}

Wang, Kefan ^{[1
,2
]}

Jia, Wendong ^{[1
,2
]}

Zhang, Panke ^{[1
]}

Huang, Shuo ^{[1
,2
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China

[2] Nanjing Univ, Chem & Biomed Innovat Ctr ChemBIC, Nanjing 210023, Peoples R China

来源：

NANO LETTERS | 2024年 / 24卷 / 38期

基金：

中国博士后科学基金; 国家重点研发计划; 中国国家自然科学基金;

关键词：

Mycobacterium smegmatis porin A; nanopore; vitamin Bs; simultaneous detection; METAL-COMPLEXES; DNA; DERIVATIVES; BIOMARKER; FOODS;

D O I：

10.1021/acs.nanolett.4c03329

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Vitamin Bs, a group of water-soluble compounds, are essential nutrients for almost all living organisms. However, due to their structural heterogeneity, rapid and simultaneous analysis of multiple vitamin Bs is still challenging. In this paper, it is discovered that a hetero-octameric Mycobacterium smegmatis porin A (MspA) nanopore containing a sole nickel ion-bound nitrilotriacetic acid (NTA-Ni) adapter at its pore constriction is suitable for the simultaneous sensing of different vitamin Bs, including vitamin B1 (thiamine), vitamin B3 (nicotinic acid and nicotinamide), vitamin B5 (pantothenic acid), and vitamin B6 (pyridoxine, pyridoxal, and pyridoxamine). Assisted by a custom machine learning algorithm, all seven vitamin Bs can be fully distinguished, reporting a general accuracy of 99.9%. This method was further validated in the rapid analysis of commercial cosmetics and natural food, suggesting its potential uses in food and drug administration.

引用

页码：11944 / 11953

页数：10

共 72 条

[1] Microbial cell factories for the sustainable manufacturing of B vitamins [J].