VvbHLH036, a basic helix-loop-helix transcription factor regulates the cold tolerance of grapevine

被引：3

作者：

Hou, Yujun ^{[1
,2
]}

Wong, Darren Chern Jan ^{[3
,4
]}

Sun, Xiaoming ^{[1
]}

Li, Qingyun ^{[1
,2
]}

Zhou, Huimin ^{[1
,2
]}

Meng, Lin ^{[1
]}

Liao, Xiaoli ^{[1
]}

Liang, Zhenchang ^{[5
,6
]}

Aryal, Rishi ^{[7
]}

Wang, Qingfeng ^{[1
]}

Xin, Haiping ^{[1
]}

机构：

[1] Chinese Acad Sci, Ctr Econ Bot, Key Lab Plant Germplasm Enhancement & Specialty A, CAS Key Lab Plant Germplasm Enhancement & Specialt, Wuhan 430074, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Australian Natl Univ, Ecol & Evolut Res Sch Biol, Acton, ACT 2601, Australia

[4] Univ Adelaide, Sch Agr Food & Wine Waite Res Precinct, Glen Osmond, SA 5064, Australia

[5] Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China

[6] Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China

[7] North Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA

来源：

PLANT PHYSIOLOGY | 2024年 / 196卷 / 04期

基金：

中国国家自然科学基金;

关键词：

CYSTATHIONINE-GAMMA-SYNTHASE; THREONINE SYNTHASE; AMINO-ACID; GENE-EXPRESSION; ANTHOCYANIN ACCUMULATION; FREEZING TOLERANCE; LOW-TEMPERATURE; STRESS; ARABIDOPSIS; ACCLIMATION;

D O I：

10.1093/plphys/kiae483

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cold stress is an adverse environmental factor that limits the growth and productivity of horticulture crops such as grapes (Vitis vinifera). In this study, we identified a grapevine cold-induced basic helix-loop-helix (bHLH) transcription factor (VvbHLH036). Overexpression and CRISPR/Cas9-mediated knockout (KO) of VvbHLH036 enhanced and decreased cold tolerance in grapevine roots, respectively. Transcriptome analysis of VvbHLH036-overexpressed roots identified threonine synthase (VvThrC1) as a potential downstream target of VvbHLH036. We confirmed that VvbHLH036 could bind the VvThrC1 promoter and activate its expression. Both the transcripts of VvThrC1 and the content of threonine were significantly induced in the leaves and roots of grapevine under cold treatment compared to controls. Conversely, these dynamics were significantly suppressed in the roots of CRISPR/Cas9-induced KO of VvbHLH036. These observations support the regulation of threonine accumulation by VvbHLH036 through VvThrC1 during cold stress in grapevine. Furthermore, overexpression and CRISPR/Cas9-mediated KO of VvThrC1 also confirmed its role in regulating threonine content and cold tolerance in transgenic roots at low temperature. Exogenous threonine treatment increased cold tolerance and reduced the accumulation of superoxide anions and hydrogen peroxide in grapevine leaves. Together, these findings point to the pivotal role of VvbHLH036 and VvThrC1 in the cold stress response in grapes by regulating threonine biosynthesis. A basic helix-loop-helix transcription factor, VvbHLH036, improves the cold tolerance of grapevine through VvThrC-activated threonine biosynthesis.

引用

页码：2871 / 2889

页数：19