Determination of ammonium and nitrate in soils by digital colorimetry

被引:2
作者
Garmay, Andrey V. [1 ]
Oskolok, Kirill V. [1 ]
Monogarova, Oksana V. [1 ]
Demidov, Mikhail I. [1 ]
机构
[1] Lomonosov Moscow State Univ, Chem Dept, Leninskie Gory 1 Build 3, Moscow 119991, Russia
关键词
Digital colorimetry; Soil; Nitrate; Ammonium; NITROGEN; NITRITE; WATER;
D O I
10.1007/s10661-024-13068-1
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A method of digital colorimetric determination of ammonium and nitrate in soils is proposed. The method is based on corresponding photometric procedures of ammonium and nitrate determination after potassium chloride extraction from soil samples. Ammonium is determined as an indophenol dye, and nitrate is determined as an azo dye. The original procedures were modified to overcome the lower sensitivity of the digital colorimetric method. For ammonium determination, the time required for the reaction to proceed completely was studied. Along with the use of a 96-well microplate protected from ambient light by a special frame, mathematical correction of scattered radiation using black ink and taking the images by a scanner in transmission mode without any post-processing, the resulting colorimetric methods proved to provide accuracy and sensitivity close to those of the spectrophotometric method, and the overall analysis speed for tens of samples was even higher. Limits of detection and quantitation for NO3- were 0.42 and 1.4 mg/kg, and for NH4+, they were 1.1 and 3.7 mg/kg, which is lower than for standard methods. The methods' validity was proven by the analysis of standard samples and by the analysis of soil samples collected in several districts of the Moscow region.
引用
收藏
页数:11
相关论文
共 50 条
[31]   Fe(II) Oxidation Shaped Functional Genes and Bacteria Involved in Denitrification and Dissimilatory Nitrate Reduction to Ammonium from Different Paddy Soils [J].
Pan, Dandan ;
Chen, Pengcheng ;
Yang, Guang ;
Niu, Rumiao ;
Bai, Yan ;
Cheng, Kuan ;
Huang, Guoyong ;
Liu, Tongxu ;
Li, Xiaomin ;
Li, Fangbai .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2023, 57 (50) :21156-21167
[32]   Adsorption of nitrate, nitrite, and ammonium ions on carbon adsorbents [J].
Gierak, Andrzej ;
Lazarska, Iwona .
ADSORPTION SCIENCE & TECHNOLOGY, 2017, 35 (7-8) :721-727
[33]   Identification of microRNAs in rice root in response to nitrate and ammonium [J].
Hua Li ;
Bin Hu ;
Wei Wang ;
Zhihua Zhang ;
Yan Liang ;
Xiaokai Gao ;
Peng Li ;
Yongqiang Liu ;
Lianhe Zhang ;
Chengcai Chu .
JournalofGeneticsandGenomics, 2016, 43 (11) :651-661
[34]   A COMPARISON OF AMMONIUM AND NITRATE AS NITROGEN-SOURCES FOR PHOTOLITHOTROPHS [J].
RAVEN, JA ;
WOLLENWEBER, B ;
HANDLEY, LL .
NEW PHYTOLOGIST, 1992, 121 (01) :19-32
[35]   Identification of microRNAs in rice root in response to nitrate and ammonium [J].
Li, Hua ;
Hu, Bin ;
Wang, Wei ;
Zhang, Zhihua ;
Liang, Yan ;
Gao, Xiaokai ;
Li, Peng ;
Liu, Yongqiang ;
Zhang, Lianhe ;
Chu, Chengcai .
JOURNAL OF GENETICS AND GENOMICS, 2016, 43 (11) :651-661
[36]   Effects of ammonium and nitrate on encystment and growth of Scrippsiella trochoidea [J].
Wang, Zhifu ;
Yu, Zhiming ;
Song, Xiuxian ;
Cao, Xihua ;
Zhang, Yue .
CHINESE SCIENCE BULLETIN, 2014, 59 (33) :4491-4497
[37]   Development of Simultaneous Ion-Exclusion/Anion-Exchange Chromatographic Determination of Ammonium, Nitrite and Nitrate Ions and Its Application to High-Salt Content Sample [J].
Mori, Masanobu ;
Hironaga, Takahiro ;
Satori, Tatsuya ;
Itabashi, Hideyuki ;
Nakatani, Nobutake ;
Kozaki, Daisuke ;
Tanaka, Kazuhiko .
TETSU TO HAGANE-JOURNAL OF THE IRON AND STEEL INSTITUTE OF JAPAN, 2011, 97 (05) :A273-A278
[38]   Comparison of Nitrate and Ammonium Leaching of Soils Collected from Different Regions of China: A Soil Column Experiment [J].
Xin Jun Dai ;
Xun Xiao ;
Wen Tai Dai ;
Shu Han Liu ;
Xiao Ying Dong ;
Ren Fang Shen ;
Xue Qiang Zhao .
Journal of Soil Science and Plant Nutrition, 2023, 23 :6059-6070
[39]   Practical considerations for the segmented-flow analysis of nitrate and ammonium in seawater and the avoidance of matrix effects [J].
Rho, Tae Keun ;
Coverly, Stephen ;
Kim, Eun-Soo ;
Kang, Dong-Jin ;
Kahng, Sung-Hyun ;
Na, Tae-Hee ;
Cho, Sung-Rok ;
Lee, Jung-Moo ;
Moon, Cho-Rong .
OCEAN SCIENCE JOURNAL, 2015, 50 (04) :709-720
[40]   Determination of ascorbic acid in natural fruit juices using digital image colorimetry [J].
Porto, Icaro S. A. ;
Santos Neto, Joao H. ;
dos Santos, Liz O. ;
Gomes, Adriano A. ;
Ferreira, Sergio L. C. .
MICROCHEMICAL JOURNAL, 2019, 149