Multicenter Study Evaluating the Vitek MS System for Identification of Medically Important Yeasts

被引:79
作者
Westblade, Lars F. [1 ,2 ]
Jennemann, Rebecca [3 ]
Branda, John A. [4 ,5 ]
Bythrow, Maureen [6 ]
Ferraro, Mary Jane [4 ,5 ]
Garner, Omai B.
Ginocchio, Christine C. [2 ,6 ]
Lewinski, Michael A. [7 ]
Manji, Ryhana [6 ]
Mochon, A. Brian [7 ]
Procop, Gary W. [8 ]
Richter, Sandra S. [8 ]
Rychert, Jenna A. [4 ,5 ]
Sercia, Linda [8 ]
Burnhama, Carey-Ann D. [1 ]
机构
[1] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63130 USA
[2] Hofstra North Shore LIJ Sch Med, Dept Pathol & Lab Med, Hempstead, NY USA
[3] Barnes Jewish Hosp, St Louis, MO 63110 USA
[4] Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
[5] Harvard Univ, Sch Med, Boston, MA USA
[6] North Shore LIJ Hlth Syst Labs, Dept Pathol & Lab Med, Lake Success, NY USA
[7] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA
[8] Cleveland Clin, Dept Clin Pathol, Cleveland, OH 44106 USA
关键词
DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; INVASIVE FUNGAL-INFECTIONS; CRYPTOCOCCUS-GATTII; CANDIDIASIS; CANDIDEMIA; EXPERIENCE; MANAGEMENT; STRATEGY; THERAPY;
D O I
10.1128/JCM.00680-13
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The optimal management of fungal infections is correlated with timely organism identification. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is revolutionizing the identification of yeasts isolated from clinical specimens. We present a multicenter study assessing the performance of the Vitek MS system (bioMerieux) in identifying medically important yeasts. A collection of 852 isolates was tested, including 20 Candida species (626 isolates, including 58 C. albicans, 62 C. glabrata, and 53 C. krusei isolates), 35 Cryptococcus neoformans isolates, and 191 other clinically relevant yeast isolates; in total, 31 different species were evaluated. Isolates were directly applied to a target plate, followed by a formic acid overlay. Mass spectra were acquired using the Vitek MS system and were analyzed using the Vitek MS v2.0 database. The gold standard for identification was sequence analysis of the D2 region of the 26S rRNA gene. In total, 823 isolates (96.6%) were identified to the genus level and 819 isolates (96.1%) were identified to the species level. Twenty-four isolates (2.8%) were not identified, and five isolates (0.6%) were misidentified. Misidentified isolates included one isolate of C. albicans (n = 58) identified as Candida dubliniensis, one isolate of Candida parapsilosis (n = 73) identified as Candida pelliculosa, and three isolates of Geotrichum klebahnii (n = 6) identified as Geotrichum candidum. The identification of clinically relevant yeasts using MS is superior to the phenotypic identification systems currently employed in clinical microbiology laboratories.
引用
收藏
页码:2267 / 2272
页数:6
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