Collagen Type II-Based Injectable Materials for In situ Repair and Regeneration of Articular Cartilage Defect

被引:1
作者
Zhang, Zhen [1 ]
Hu, Xu [1 ]
Jin, Min [1 ,2 ]
Mu, Yulei [1 ]
Zhou, Huiqun [1 ,2 ]
Ma, Cheng [1 ,2 ]
Ma, Liang [1 ]
Liu, Bangheng [1 ,2 ]
Yao, Hang [3 ]
Huang, Ye [4 ]
Wang, Dong-An [1 ,2 ,5 ]
机构
[1] City Univ Hong Kong, Dept Biomed Engn, Hong Kong, Peoples R China
[2] HKSTP, Karolinska Inst Ming Wai Lau Ctr Reparat Med, Sha Tin, Hong Kong, Peoples R China
[3] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou, Peoples R China
[4] Beiiing Jishuitan Hosp, Knee Preservat Clin & Res Ctr, Beijing, Peoples R China
[5] HKSTP, Ctr Neuromusculoskeletal Restorat Med, Hong Kong, Peoples R China
关键词
CHONDROGENIC DIFFERENTIATION; STEM-CELLS; HYDROGELS; BONE; CHONDROCYTES; OSTEOARTHRITIS; DEGRADATION; SCAFFOLDS; MATRICES; DESIGN;
D O I
10.34133/bmr.0072
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Repairing and regenerating articular cartilage defects (ACDs) have long been challenging for physicians and scientists. The rise of injectable materials provides a novel strategy for minimally invasive surgery to repair ACDs. In this study, we successfully developed injectable materials based on collagen type II, achieving hyaline cartilage repair and regeneration of ACDs. Analysis was conducted on the regenerated cartilage after materials injection. The histology staining demonstrated complete healing of the ACDs with the attainment of a hyaline cartilage phenotype. The biochemical and biomechanical properties are similar to the adjacent native cartilage without noticeable adverse effects on the subchondral bone. Further transcriptome analysis found that compared with the Native cartilage adjacent to the defect area, the Regenerated cartilage in the defect area repaired with type II collagen-based injection materials showed changes in cartilage-related pathways, as well as down-regulation of T cell receptor signaling pathways and interleukin-17 signaling pathways, which changed the immune microenvironment of the ACD area. Overall, these findings offer a promising injectable approach to treating ACDs, providing a potential solution to the challenges associated with achieving hyaline cartilage in situ repair and regeneration while minimizing damage to the surrounding cartilage.
引用
收藏
页数:18
相关论文
共 48 条
[1]   The role of chondroitin sulfate in regulating hypertrophy during MSC chondrogenesis in a cartilage mimetic hydrogel under dynamic loading [J].
Aisenbrey, Elizabeth A. ;
Bryant, Stephanie J. .
BIOMATERIALS, 2019, 190 :51-62
[2]   Cdkn1c (p57Kip2) is the major regulator of embryonic growth within its imprinted domain on mouse distal chromosome 7 [J].
Andrews, Stuart C. ;
Wood, Michelle D. ;
Tunster, Simon J. ;
Barton, Sheila C. ;
Surani, M. Azim ;
John, Rosalind M. .
BMC DEVELOPMENTAL BIOLOGY, 2007, 7
[3]   Collagen Type II enhances chondrogenic differentiation in agarose-based modular microtissues [J].
Annamalai, Ramkumar Tiruvannamalai ;
Mertz, David R. ;
Daley, Ethan L. H. ;
Stegemann, Jan P. .
CYTOTHERAPY, 2016, 18 (02) :263-277
[4]   In Situ D-periodic Molecular Structure of Type II Collagen [J].
Antipova, Olga ;
Orgel, Joseph P. R. O. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (10) :7087-7096
[5]   Redifferentiated Chondrocytes in Fibrin Gel for the Repair of Articular Cartilage Lesions [J].
Bianchi, Vanessa J. ;
Lee, Adrienne ;
Anderson, Jesse ;
Parreno, Justin ;
Theodoropoulos, John ;
Backstein, David ;
Kandel, Rita .
AMERICAN JOURNAL OF SPORTS MEDICINE, 2019, 47 (10) :2348-2359
[6]   Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: Influence of collagen type II extracellular matrix on MSC chondrogenesis [J].
Bosnakovski, D ;
Mizuno, M ;
Kim, G ;
Takagi, S ;
Okumura, M ;
Fujinaga, T .
BIOTECHNOLOGY AND BIOENGINEERING, 2006, 93 (06) :1152-1163
[7]   Histological evaluation of the course of healing of canine articular cartilage defects treated with cultured autologous chondrocytes [J].
Breinan, HA ;
Minas, T ;
Barone, L ;
Tubo, R ;
Hsu, HP ;
Shortkroff, S ;
Nehrer, S ;
Sledge, CB ;
Spector, M .
TISSUE ENGINEERING, 1998, 4 (01) :101-114
[8]   Cross-linked type I and type II collagenous matrices for the repair of full-thickness articular cartilage defects - A study in rabbits [J].
Buma, P ;
Pieper, JS ;
van Tienen, T ;
van Susante, JLC ;
van der Kraan, PM ;
Veerkamp, JH ;
van den Berg, WB ;
Veth, RPH ;
van Kuppevelt, TH .
BIOMATERIALS, 2003, 24 (19) :3255-3263
[9]   A Novel Design of Multi-Mechanoresponsive and Mechanically Strong Hydrogels [J].
Chen, Hong ;
Yang, Fengyu ;
Chen, Qiang ;
Zheng, Jie .
ADVANCED MATERIALS, 2017, 29 (21)
[10]   Global, regional prevalence, incidence and risk factors of knee osteoarthritis in population-based studies [J].
Cui, Aiyong ;
Li, Huizi ;
Wang, Dawei ;
Zhong, Junlong ;
Chen, Yufeng ;
Lu, Huading .
ECLINICALMEDICINE, 2020, 29-30