TERT upregulation promotes cell proliferation via degradation of p21 and increases carcinogenic potential

被引：3

作者：

Mishima, Masako ^{[1
]}

Takai, Atsushi ^{[1
]}

Takeda, Haruhiko ^{[1
]}

Iguchi, Eriko ^{[1
]}

Nakano, Shigeharu ^{[1
]}

Fujii, Yosuke ^{[1
]}

Ueno, Masayuki ^{[1
]}

Ito, Takahiko ^{[1
]}

Teramura, Mari ^{[1
]}

Eso, Yuji ^{[1
]}

Shimizu, Takahiro ^{[1
]}

Maruno, Takahisa ^{[1
]}

Hidema, Shizu ^{[2
]}

Nishimori, Katsuhiko ^{[2
]}

Marusawa, Hiroyuki ^{[3
]}

Hatano, Etsuro ^{[4
]}

Seno, Hiroshi ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Gastroenterol & Hepatol, 54 Kawahara cho,Shogoin,Sakyo Ku, Kyoto 6068507, Japan

[2] Fukushima Med Univ, Dept Bioregulat & Pharmacol Med, Fukushima, Japan

[3] Osaka Red Cross Hosp, Dept Gastroenterol & Hepatol, Osaka, Japan

[4] Kyoto Univ, Grad Sch Med, Dept Surg, Kyoto, Japan

来源：

JOURNAL OF PATHOLOGY | 2024年 / 264卷 / 03期

基金：

日本学术振兴会;

关键词：

hepatocellular carcinoma; cell cycle; liver cancer; carcinogenesis; hepatocyte; tert; mouse models; REVERSE-TRANSCRIPTASE; HEPATOCELLULAR-CARCINOMA; MUTATIONAL LANDSCAPE; TELOMERASE; CANCER; P21(CIP1/WAF1); EXPRESSION; SCFSKP2; PATHWAY; LIVER;

D O I：

10.1002/path.6351

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Telomerase reverse transcriptase (TERT) gene aberration is detectable in >80% of cases with hepatocellular carcinoma (HCC). TERT reactivation is essential for cellular immortalization because it stabilizes telomere length, although the role of TERT in hepatocarcinogenesis remains unelucidated. To elucidate the significance of aberrant TERT expression in hepatocytes in inflammation-associated hepatocarcinogenesis, we generated Alb-Cre;TertTg mice, which overexpress TERT in the liver and examined their phenotype during chronic inflammation. Based on transcriptome data from the liver tissue of Alb-Cre;TertTg mice, we examined the role of TERT in hepatocarcinogenesis in vitro. We also evaluated the relationship between TERT and cell-cycle-related molecules, including p21, in HCC samples. The liver tumor development rate was increased by TERT overexpression during chronic inflammation, especially in the absence of p53 function. Gene set enrichment analysis of liver tissues revealed that gene sets related to TNF-NF kappa B signaling, cell cycle, and apoptosis were upregulated in Alb-Cre;TertTg liver. A luciferase reporter assay and immunoprecipitation revealed that TERT interacted with NF kappa B p65 and enhanced NFKB1 promoter activity. On the other hand, TERT formed protein complexes with p21, cyclin A2, and cyclin E and promoted ubiquitin-mediated degradation of p21, specifically in the G1 phase. In the clinical HCC samples, TERT was highly expressed but p21 was conversely downregulated, and TERT expression was associated with the upregulation of molecules related to the cell cycle. Taken together, the aberrant upregulation of TERT increased NFKB1 promoter activity and promoted cell cycle progression via p21 ubiquitination, leading to hepatocarcinogenesis. (c) 2024 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.

引用

页码：318 / 331

页数：14

共 48 条

[1] p21 in cancer: intricate networks and multiple activities [J].