S. enteritidis Genome Loads in the Tissues of Reproductive Organs in Laying Duck

被引：0

作者：

He, Guang-Zhi ^{[1
]}

Deng, Shu-Xuan ^{[2
]}

机构：

[1] Guiyang Coll Tradit Chinese Med, Guiyang 550002, Guizhou Provinc, Peoples R China

[2] Agr Off Dalingshan Town, Dongguan 523830, Guandong Provin, Peoples R China

来源：

JOURNAL OF ANIMAL AND VETERINARY ADVANCES | 2011年 / 10卷 / 23期

关键词：

Salmonella enteritidis; reproductive organs; duck; PCR; China; ENTERICA SEROVAR ENTERITIDIS; REAL-TIME PCR; SALMONELLA-ENTERITIDIS; ORAL CHALLENGE; EGG CONTAMINATION; TRACT; COLONIZATION; INVASION; OVIDUCT; HENS;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The objective of this study was to determine the replication kinetics of Salmonella enteritidis genome loads (S. enteritidis) in. reproductive organs of laying duck after oral challenge. We conducted serovar-specific real-time PCR for S. enteritidis to detect the genomic DNA of S. enteritidis in laying duck at different time points. To validate these results, the Indirect Fluorescent Antibody (IFA) technique was employed too. The results showed that S. enteritidis was consistently detected in all the samples. However, the number of bacteria started decreasing by 3-4 days and by 6 days, the concentration of S. enteritidis DNA was below the detection limits of the PCR assay except the spleen and vagina. In conclusion, this study will help in understanding the pathogenesis of S. enteritidis infection in vivo.

引用

页码：3165 / 3168

页数：4

共 7 条

[1] Populations of Salmonella enteritidis in the Tissues of Reproductive Organs in Laying Hens After Oral Challenge: A Real-Time PCR Study
He, Guang-Zhi
Tian, Wei-Yi
Qian, Ning
Deng, Shu-Xuan
JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2011, 10 (08): : 945 - 948
[2] Salmonella enterica serovar Enteritidis live vaccine strain in the reproductive organs of laying goose after subcutaneous vaccination
He, G. Z.
Deng, S. X.
Tian, W. Y.
IRANIAN JOURNAL OF VETERINARY RESEARCH, 2013, 14 (02) : 100 - 105
[3] Invasion of Salmonella enteritidis in the tissues of reproductive organs in laying Japanese quail:: An immunocytochemical study
Takata, T
Liang, J
Nakano, H
Yoshimura, Y
POULTRY SCIENCE, 2003, 82 (07) : 1170 - 1173
[4] Contamination of eggs of laying hens with S. Enteritidis
Jaeger, Gerald
TIERAERZTLICHE UMSCHAU, 2009, 64 (7-8) : 344 - 348
[5] The Relationship Between the Numbers of Salmonella Enteritidis, Salmonella Heidelberg, or Salmonella Hadar Colonizing Reproductive Tissues of Experimentally Infected Laying Hens and Deposition Inside Eggs
Gast, Richard K.
Guraya, Rupa
Guard, Jean
Holt, Peter S.
AVIAN DISEASES, 2011, 55 (02) : 243 - 247
[6] Colonization of reproductive organs and internal contamination of eggs after experimental infection of laying hens with Salmonella heidelberg and Salmonella enteritidis
Gast, PK
Guard-Bouldin, J
Holt, PS
AVIAN DISEASES, 2004, 48 (04) : 863 - 869
[7] The Distributions of S. enteritidis in the Internal Organs of Chinese Guizhou Minipig after Orally Infected
He, Guang-Zhi
Tian, Wei-Yi
Qian, Ning
Deng, Shu-Xuan
JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2011, 10 (17): : 2275 - 2277

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