Liquid Biopsy Instrument for Ultra-Fast and Label-Free Detection of Circulating Tumor Cells

被引：6
作者：
Zhu, Shu [1 ,2 ,3 ,4 ]
Zhu, Zhixian [1 ,2 ]
Ni, Chen [1 ,2 ]
Zhou, Zheng [1 ,2 ]
Chen, Yao [1 ,2 ]
Tang, Dezhi [1 ,2 ]
Guo, Kefan [1 ,2 ]
Yang, Shuai [1 ,2 ]
Liu, Kang [1 ,2 ]
Ni, Zhonghua [1 ,2 ]
Xiang, Nan [1 ,2 ]
机构：
[1] Southeast Univ, Sch Mech Engn, Nanjing 211189, Peoples R China
[2] Southeast Univ, Jiangsu Key Lab Design & Manufacture Micronano Bi, Nanjing 211189, Peoples R China
[3] Nanjing Normal Univ, Sch Elect & Automat Engn, Nanjing 210023, Peoples R China
[4] Nanjing Normal Univ, Jiangsu Key Lab 3D Printing Equipment & Mfg, Nanjing 210023, Peoples R China
来源：
RESEARCH | 2024年 / 7卷
关键词：
Blood - Cells - Cytology - Deep learning - Dielectric properties - Diseases - Tumors;
D O I：
10.34133/research.0431
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Rapid diagnosis and real-time monitoring are of great important in the fight against cancer. However, most available diagnostic technologies are time-consuming and labor-intensive and are commonly invasive. Here, we describe CytoExam, an automatic liquid biopsy instrument designed based on inertial microfluidics and impedance cytometry, which uses a deep learning algorithm for the analysis of circulating tumor cells (CTCs). In silico and in vitro experiments demonstrated that CytoExam could achieve label-free detection of CTCs in the peripheral blood of cancer patients within 15 min. The clinical applicability of CytoExam was also verified using peripheral blood samples from 10 healthy donors and >50 patients with breast, colorectal, or lung cancer. Significant differences in the number of collected cells and predicted CTCs were observed between the 2 groups, with variations in the dielectric properties of the collected cells from cancer patients also being observed. The ultra-fast and minimally invasive features of CytoExam may pave the way for new paths for cancer diagnosis and scientific research.
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页数：12
相关论文
共 47 条
[1]  
Ahmed S, 2023, J CLIN ONCOL, V41
[2]   Counting circulating endothelial cells in allo-HSCT: an ad hoc designed polychromatic flowcytometry-based panel versus the CellSearch System [J].
Almici, Camillo ;
Neva, Arabella ;
Skert, Cristina ;
Bruno, Benedetto ;
Verardi, Rosanna ;
Di Palma, Andrea ;
Bianchetti, Andrea ;
Braga, Simona ;
Piovani, Giovanna ;
Cancelli, Valeria ;
Omede, Paola ;
Baeten, Kurt ;
Rotta, Gianluca ;
Russo, Domenico ;
Marini, Mirella .
SCIENTIFIC REPORTS, 2019, 9 (1)
[3]  
Barbato C, 2022, AM J CLIN PATHOL, V158, pS50
[4]   Viscoelastic-Sorting Integrated Deformability Cytometer for High-Throughput Sorting and High-Precision Mechanical Phenotyping of Tumor Cells [J].
Chen, Yao ;
Jiang, Lin ;
Zhang, Xiaozhe ;
Ni, Zhonghua ;
Xiang, Nan .
ANALYTICAL CHEMISTRY, 2023, 95 (49) :18180-18187
[5]   Ultrasensitive label-free detection of circulating tumor cells using conductivity matching of two-dimensional semiconductor with cancer cell [J].
Chen, Yuanyuan ;
Peng, Jian ;
Lai, Youqun ;
Wu, Binghui ;
Sun, Liping ;
Weng, Jian .
BIOSENSORS & BIOELECTRONICS, 2019, 142
[6]   Detection and localization of surgically resectable cancers with a multi-analyte blood test [J].
Cohen, Joshua D. ;
Li, Lu ;
Wang, Yuxuan ;
Thoburn, Christopher ;
Afsari, Bahman ;
Danilova, Ludmila ;
Douville, Christopher ;
Javed, Ammar A. ;
Wong, Fay ;
Mattox, Austin ;
Hruban, Ralph. H. ;
Wolfgang, Christopher L. ;
Goggins, Michael G. ;
Dal Molin, Marco ;
Wang, Tian-Li ;
Roden, Richard ;
Klein, Alison P. ;
Ptak, Janine ;
Dobbyn, Lisa ;
Schaefer, Joy ;
Silliman, Natalie ;
Popoli, Maria ;
Vogelstein, Joshua T. ;
Browne, James D. ;
Schoen, Robert E. ;
Brand, Randall E. ;
Tie, Jeanne ;
Gibbs, Peter ;
Wong, Hui-Li ;
Mansfield, Aaron S. ;
Jen, Jin ;
Hanash, Samir M. ;
Falconi, Massimo ;
Allen, Peter J. ;
Zhou, Shibin ;
Bettegowda, Chetan ;
Diaz, Luis A., Jr. ;
Tomasetti, Cristian ;
Kinzler, Kenneth W. ;
Vogelstein, Bert ;
Lennon, Anne Marie ;
Papadopoulos, Nickolas .
SCIENCE, 2018, 359 (6378) :926-+
[7]   Combined circulating tumor DNA and protein biomarker-based liquid biopsy for the earlier detection of pancreatic cancers [J].
Cohen, Joshua D. ;
Javed, Ammar A. ;
Thoburn, Christopher ;
Wong, Fay ;
Tie, Jeanne ;
Gibbs, Peter ;
Schmidt, C. Max ;
Yip-Schneider, Michele T. ;
Allen, Peter J. ;
Schattner, Mark ;
Brand, Randall E. ;
Singhi, Aatur D. ;
Petersen, Gloria M. ;
Hong, Seung-Mo ;
Kim, Song Cheol ;
Falconi, Massimo ;
Doglioni, Claudio ;
Weiss, Matthew J. ;
Ahuja, Nita ;
He, Jin ;
Makary, Martin A. ;
Maitra, Anirban ;
Hanash, Samir M. ;
Dal Molin, Marco ;
Wang, Yuxuan ;
Li, Lu ;
Ptak, Janine ;
Dobbyn, Lisa ;
Schaefer, Joy ;
Silliman, Natalie ;
Popoli, Maria ;
Goggins, Michael G. ;
Hruban, Ralph H. ;
Wolfgang, Christopher L. ;
Klein, Alison P. ;
Tomasetti, Cristian ;
Papadopoulos, Nickolas ;
Kinzler, Kenneth W. ;
Vogelstein, Bert ;
Lennon, Anne Marie .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (38) :10202-10207
[8]   Liquid biopsy versus tumor biopsy for clinical-trial recruitment [J].
Corcoran, Ryan B. .
NATURE MEDICINE, 2020, 26 (12) :1815-1816
[9]   Impedance-Based Multimodal Electrical-Mechanical Intrinsic Flow Cytometry [J].
Feng, Yongxiang ;
Zhu, Junwen ;
Chai, Huichao ;
He, Weihua ;
Huang, Liang ;
Wang, Wenhui .
SMALL, 2023, 19 (45)
[10]   Apoptotic Bodies in the Pancreatic Tumor Cell Culture Media Enable Label-Free Drug Sensitivity Assessment by Impedance Cytometry [J].
Honrado, Carlos ;
Adair, Sara J. ;
Moore, John H. ;
Salahi, Armita ;
Bauer, Todd W. ;
Swami, Nathan S. .
ADVANCED BIOLOGY, 2021, 5 (08)
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