Proteomic Profiling of Cocos nucifera L. Zygotic Embryos during Maturation of Dwarf and Tall Cultivars: The Dynamics of Carbohydrate and Fatty Acid Metabolism

被引:0
|
作者
Granados-Alegria, Maria Ines [1 ]
Canto-Canche, Blondy [2 ]
Gomez-Tah, Rufino [2 ]
Felix, Jean Wildort [1 ]
Tzec-Sima, Miguel [1 ]
Ruiz-May, Eliel [3 ]
Islas-Flores, Ignacio [1 ]
机构
[1] Ctr Invest Cient Yucatan AC, Unidad Biol Integrat, Calle 43 130 X 32 Y 34, Merida 97205, Yucatan, Mexico
[2] Ctr Invest Cient Yucatan AC, Unidad Biotecnol, Calle 43 130 X 32 Y 34, Merida 97205, Yucatan, Mexico
[3] Inst Ecol AC, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, Xalapa 91073, Veracruz, Mexico
关键词
Cocos nucifera; embryo development; zygotic embryo; solid endosperm; TMT proteomics; PROTEIN; DETOXIFICATION; METHYLGLYOXAL; ARABIDOPSIS; EXPRESSION; ENZYME;
D O I
10.3390/ijms25158507
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
There is a limited number of studies analyzing the molecular and biochemical processes regulating the metabolism of the maturation of Cocos nucifera L. zygotic embryos. Our research focused on the regulation of carbohydrate and lipid metabolic pathways occurring at three developmental stages of embryos from the Mexican Pacific tall (MPT) and the Yucatan green dwarf (YGD) cultivars. We used the TMT-synchronous precursor selection (SPS)-MS3 strategy to analyze the dynamics of proteomes from both embryos; 1044 and 540 proteins were determined for the MPT and YGD, respectively. A comparison of the differentially accumulated proteins (DAPs) revealed that the biological processes (BP) enriched in the MPT embryo included the glyoxylate and dicarboxylate metabolism along with fatty acid degradation, while in YGD, the nitrogen metabolism and pentose phosphate pathway were the most enriched BPs. Findings suggest that the MPT embryos use fatty acids to sustain a higher glycolytic/gluconeogenic metabolism than the YGD embryos. Moreover, the YGD proteome was enriched with proteins associated with biotic or abiotic stresses, e.g., peroxidase and catalase. The goal of this study was to highlight the differences in the regulation of carbohydrate and lipid metabolic pathways during the maturation of coconut YGD and MPT zygotic embryos.
引用
收藏
页数:24
相关论文
共 11 条
  • [1] Lauric acid improves the growth of zygotic coconut (Cocos nucifera L.) embryos in vitro
    Arturo López-Villalobos
    Peter F. Dodds
    Roland Hornung
    Plant Cell, Tissue and Organ Culture (PCTOC), 2011, 106 : 317 - 327
  • [2] Lauric acid improves the growth of zygotic coconut (Cocos nucifera L.) embryos in vitro
    Lopez-Villalobos, Arturo
    Dodds, Peter F.
    Hornung, Roland
    PLANT CELL TISSUE AND ORGAN CULTURE, 2011, 106 (02) : 317 - 327
  • [3] Photosynthetic irradiance response, canopy photosynthesis and their variation with canopy strata in tall and dwarf x tall coconut cultivars (Cocos nucifera L.)
    Ranasinghe, C. S.
    Kumarathunge, M. D. P.
    Jayaranjini, S.
    De Costa, W. A. J. M.
    SCIENTIA HORTICULTURAE, 2015, 189 : 175 - 183
  • [4] Physicochemical characteristics of kernel during fruit maturation of four coconut cultivars (Cocos nucifera L.)
    Rachel, Assa Rebecca
    Jean-Louis, Konan Konan
    Alexia, Prades
    Jean, Nemlin
    Ernest, Koffi
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2010, 9 (14): : 2136 - 2144
  • [5] Changes in photosynthetic parameters during in vitro growth and subsequent acclimatization of coconut (Cocos nucifera L.) zygotic embryos
    Triques, K
    Rival, A
    Beule, T
    Morcillo, F
    Hocher, V
    Verdeil, JL
    Hamon, S
    INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY OF TROPICAL AND SUBTROPICAL SPECIES - PART II, 1998, (461): : 275 - 282
  • [6] Changes in fatty acid composition during development of tissues of coconut (Cocos nucifera L.) embryos in the intact nut and in vitro
    López-Villalobos, A
    Dodds, PF
    Hornung, R
    JOURNAL OF EXPERIMENTAL BOTANY, 2001, 52 (358) : 933 - 942
  • [7] GA3 induces expression of E2F-like genes and CDKA during in vitro germination of zygotic embryos of Cocos nucifera (L.)
    Montero-Cortes, Mayra
    Cordova, Ivan
    Verdeil, Jean-Luc
    Hocher, Valerie
    Pech y Ake, America
    Sandoval, Alfredo
    Oropeza, Carlos
    Saenz, Luis
    PLANT CELL TISSUE AND ORGAN CULTURE, 2011, 107 (03) : 461 - 470
  • [8] GA3 induces expression of E2F-like genes and CDKA during in vitro germination of zygotic embryos of Cocos nucifera (L.)
    Mayra Montero-Cortés
    Iván Cordova
    Jean-Luc Verdeil
    Valerie Hocher
    América Pech y Ake
    Alfredo Sandoval
    Carlos Oropeza
    Luis Sáenz
    Plant Cell, Tissue and Organ Culture (PCTOC), 2011, 107 : 461 - 470
  • [9] Expression profiling of cell cycle regulatory genes E2F and CDKA during early stages of zygotic embryo culture in coconut (Cocos nucifera L.)
    K. Mounika Sai
    Smita Nair
    Megha Mohan
    A. G. Kiran
    Plant Cell, Tissue and Organ Culture (PCTOC), 2023, 155 : 709 - 717
  • [10] Expression profiling of cell cycle regulatory genes E2F and CDKA during early stages of zygotic embryo culture in coconut (Cocos nucifera L.)
    Sai, K. Mounika
    Nair, Smita
    Mohan, Megha
    Kiran, A. G.
    PLANT CELL TISSUE AND ORGAN CULTURE, 2023, 155 (03) : 709 - 717