Asiatic acid reduces lipopolysaccharides-induced pulp inflammation fl ammation through activation of nuclear factor erythroid 2-related factor 2 in rats

Background: Dental pulp inflammation, fl ammation, often initiated by Gram-negative microorganisms and lipopolysaccharides (LPS), can lead to pulpitis and, subsequently, dental pulp necrosis, compromising tooth structure and increasing susceptibility to fracture. Asiatic acid, derived from Centella asiatica, , has demonstrated pharmacological properties, including anti-inflammatory fl ammatory and antioxidant effects, making it a potential candidate for mitigating LPS-induced pulp inflammation. fl ammation. This in vivo study aims to investigate the impact of Asiatic acid on the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway in Rattus norvegicus with LPS-induced pulp inflammation. fl ammation. Methods: This quasi-laboratory experimental in vivo study employed a post-test- only control group design to investigate the effects of Asiatic acid on LPS-induced pulp inflammation fl ammation in Wistar rats. Thirty rats were randomly divided into six groups subjected to various interventions. LPS was administered to all groups for 6 h except the standard control group (CG, n = 5). The negative control group (NCG, n = 5) received only glass ionomer cement. The positive control group (PCG, n = 5) received Eugenol with glass ionomer cement. Intervention groups 1, 2, and 3 (IG1, IG2, IG3; n = 5 each) received Asiatic acid at concentrations of 0.5%, 1%, and 2%, respectively, with glass ionomer cement. Dental pulp inflammation fl ammation was confirmed fi rmed through immunological (tumor necrosis factor alpha (TNF-a) a ) levels), histopathological (inflammatory fl ammatory parameters), and physiological (pain assessment using the rat grimace scale) analyses. Additionally, Nrf2 levels were examined using enzyme-linked immunosorbent assay (ELISA). Results: Asiatic acid administration significantly fi cantly influenced fl uenced Nrf2 levels in rats with LPS-induced pulp inflammation. fl ammation. Nrf2 levels were significantly fi cantly higher in groups treated with 0.5% (IG1) (8.810 +/- 1.092 ng/mL; p = 0.047), 1.0% (IG2) (9.132 +/- 1.285 ng/mL; p = 0.020), and 2.0% (IG3) (11.972 +/- 1.888 ng/mL; p = 0.000) Asiatic acid compared to NCG (7.146 +/- 0.706). Notably, Nrf2 levels were also significantly fi cantly higher in the 2.0% Asiatic acid group (IG3) compared to the PCG treated with Eugenol (8.846 +/- 0.888 ng/mL; p = 0.001), as well as IG1 (p p = 0.001) and IG2 (p p = 0.002). However, no significant fi cant difference was observed between administering 0.5% Asiatic acid (IG1), 1.0% Asiatic acid (IG2), and Eugenol (PCG). Conclusion: This research showed that Asiatic acid significantly fi cantly impacted the Nrf2 levels in rats with LPS-induced pulp inflammation. fl ammation. This suggests that it has the potential to be used as a therapeutic agent for reducing dental pulp inflammation. fl ammation. These fi ndings support the need to further explore Asiatic acid as a promising intervention for maintaining dental pulp health.