Exploring Differentially Expressed Sperm miRNAs in Idiopathic Recurrent Pregnancy Loss and Their Association with Early Embryonic Development

被引:3
作者
Thapliyal, Ayushi [1 ]
Tomar, Anil Kumar [1 ]
Naglot, Sarla [2 ]
Dhiman, Soniya [3 ]
Datta, Sudip Kumar [4 ]
Sharma, Jai Bhagwan [3 ]
Singh, Neeta [3 ]
Yadav, Savita [1 ]
机构
[1] All India Inst Med Sci, Dept Biophys, New Delhi 110029, India
[2] Indian Council Med Res ICMR, Div Reprod Child Hlth & Nutr, New Delhi 110029, India
[3] All India Inst Med Sci, Dept Obstet & Gynaecol, New Delhi 110029, India
[4] All India Inst Med Sci, Dept Lab Med, New Delhi 110029, India
关键词
differential expression analysis; male contributory factors; microRNA; recurrent pregnancy loss; spermatozoa; MALE-INFERTILITY; OXIDATIVE STRESS; PROTEIN-KINASES; DNA INTEGRITY; MESSENGER-RNA; HUMAN TESTIS; MISCARRIAGE; SPERMATOZOA; ANEUPLOIDY; MICRORNAS;
D O I
10.3390/ncrna10040041
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The high incidence of idiopathic recurrent pregnancy loss (iRPL) may stem from the limited research on male contributory factors. Many studies suggest that sperm DNA fragmentation and oxidative stress contribute to iRPL, but their roles are still debated. MicroRNAs (miRNAs) are short non-coding RNAs that regulate various biological processes by modulating gene expression. While differential expression of specific miRNAs has been observed in women suffering from recurrent miscarriages, paternal miRNAs remain unexplored. We hypothesize that analyzing sperm miRNAs can provide crucial insights into the pathophysiology of iRPL. Therefore, this study aims to identify dysregulated miRNAs in the spermatozoa of male partners of iRPL patients. Total mRNA was extracted from sperm samples of iRPL and control groups, followed by miRNA library preparation and high-output miRNA sequencing. Subsequently, raw sequence reads were processed for differential expression analysis, target prediction, and bioinformatics analysis. Twelve differentially expressed miRNAs were identified in the iRPL group, with eight miRNAs upregulated (hsa-miR-4454, hsa-miR-142-3p, hsa-miR-145-5p, hsa-miR-1290, hsa-miR-1246, hsa-miR-7977, hsa-miR-449c-5p, and hsa-miR-92b-3p) and four downregulated (hsa-miR-29c-3p, hsa-miR-30b-5p, hsa-miR-519a-2-5p, and hsa-miR-520b-5p). Functional enrichment analysis revealed that gene targets of the upregulated miRNAs are involved in various biological processes closely associated with sperm quality and embryonic development.
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页数:17
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