Development and Use of Human Recombinant 64Cu-rHCF as a Kidney Glomerulus-Targeted Contrast Agent for Positron Emission Tomography

被引：0
作者：
Baldelomar, Edwin J. [1 ,2 ]
Zhang, Hanwen [2 ]
Thorek, Daniel [2 ]
Charlton, Jennifer R. [3 ]
Walker, Patrick D. [4 ]
Wilson, Leslie D. [5 ]
Emoto, Kasey C. [6 ]
Jordan, Veronica Clavijo [7 ]
Reichert, David E. [2 ]
Shoghi, Kooresh [2 ]
Bennett, Kevin M. [2 ]
机构：
[1] XN Biotechnol LLC, St. Louis, MO 63110 USA
[2] Washington Univ St Louis, Mallinckrodt Inst Radiol, St. Louis, MO 63110 USA
[3] Univ Virginia, Dept Pediat, Div Nephrol, Charlottesville, VA 22904 USA
[4] Arkana Labs, Little Rock, AR 72211 USA
[5] Washington Univ St Louis, Div Comparat Med, St Louis, MO 63110 USA
[6] Univ Hawaii Manoa, Dept Biol, Honolulu, HI 96822 USA
[7] Harvard Med Sch, Massachusetts Gen Hosp, Athinoula A Martinos Ctr Biomed Imaging, Dept Radiol, Charlestown, MA 02115 USA
来源：
ACS APPLIED BIO MATERIALS | 2024年 / 7卷 / 10期
关键词：
Human Recombinant Ferritin; Positron Emission Tomography; Kidney Nephron Number; Glomerulus; CFE-MRI; CATIONIZED FERRITIN; NEPHRON NUMBER; IN-VIVO; MRI; SIZE; NANOPARTICLES; PATHOLOGY; INJURY; MICE;
D O I：
10.1021/acsabm.4c01027
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
In this work, we develop recombinant human cationic ferritin (rHCF) as a contrast agent to detect glomeruli in the kidney using positron emission tomography (PET). We first expressed recombinant human ferritin (rHF) in E. coli and then functionalized and radiolabeled it with Copper-64 (64Cu) to form Cu-64-rHCF. Intravenously injected Cu-64-rHCF bound to kidney glomeruli and was detected by PET. A subchronic toxicity study after an intravenous injection of rHCF revealed no significant toxicity. The development of rHCF is an important step toward the potential clinical translation of CF to detect the nephron number in humans.
引用
收藏
页码：6392 / 6397
页数：6
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