Proteome analysis provides insights into sex differences in Holothuria Scabra

被引：0
作者：
Cheng, Chuhang [1 ,2 ]
Wu, Feifei [3 ]
Xu, Yizhi [4 ]
Ren, Chunhua [5 ]
Chen, Ting [5 ]
Li, Shella [6 ]
Shen, Peihong [2 ]
Jiang, Fajun [1 ]
机构：
[1] Guangxi Acad Sci, Guangxi Acad Marine Sci, Guangxi Key Lab Marine Environm Sci, Nanning, Peoples R China
[2] Guangxi Univ, Coll Life Sci & Technol, Nanning, Peoples R China
[3] Sun Yat Sen Univ, Sch Marine Sci, Zhuhai, Peoples R China
[4] Univ Edinburgh, Sch Biol Sci, Edinburgh, Scotland
[5] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou, Peoples R China
[6] BASIS Int Sch, Guangzhou, Peoples R China
来源：
PLOS ONE | 2024年 / 19卷 / 08期
基金：
中国博士后科学基金;
关键词：
EXPRESSION; FERTILIZATION; PROTEINS; TESTIS; PROTEOLIAISIN; ANTIGEN; GENES; OVARY; RNA;
D O I：
10.1371/journal.pone.0301884
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Sex-determining mechanism is still ambiguous for sea cucumber Holothuria scabra which only manifests gonochorism in gonad. In this study, proteomic analysis was employed to delineate sex-related proteins and genes in gonads of H. scabra, subsequently validated through Quantitative real-time polymerase chain reaction (qRT-PCR). A total of 5,313 proteins were identified via proteome sequencing. Among these, 817 proteins exhibited expression in both the ovary and testis, with 445 proteins displaying up-regulation and 372 proteins showing down-regulation (ovary vs testis). Furthermore, 136 and 69 proteins were identified as ovary-specific and testis-specific Differentially Abundant Proteins (DAPs), respectively. And 9 DAP coding genes which play crucial role in ovary and testis were verified by qRT-PCR. Notably, 24 ovary-bias proteins enriched in ribosome pathway strongly indicated the crucial role of ribosome in ovary. This study serves to furnish novel evidence pertaining to sex differences in H. scabra.
引用
收藏
页数：16
相关论文
共 57 条
[41]   SRplot: A free online platform for data visualization and graphing [J].
Tang, Doudou ;
Chen, Mingjie ;
Huang, Xinhua ;
Zhang, Guicheng ;
Zeng, Lin ;
Zhang, Guangsen ;
Wu, Shangjie ;
Wang, Yewei .
PLOS ONE, 2023, 18 (11)
[42]   Existence and distribution of gonadotropin-releasing hormone-like peptides in the central nervous system and ovary of the Pacific white shrimp, Litopenaeus vannamei [J].
Tinikul, Yotsawan ;
Poljaroen, Jaruwan ;
Nuurai, Parinyaporn ;
Anuracpreeda, Panat ;
Chotwiwatthanakun, Charoonroj ;
Phoungpetchara, Ittipon ;
Kornthong, Napamanee ;
Poomtong, Tanes ;
Hanna, Peter J. ;
Sobhon, Prasert .
CELL AND TISSUE RESEARCH, 2011, 343 (03) :579-593
[43]   Molecular characterization and expression profiles of cyclin A and cyclin B during ovarian development of the giant tiger shrimp Penaeus monodon [J].
Visudtiphole, Virak ;
Klinbunga, Sirawut ;
Kirtikara, Kanyawim .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2009, 152 (04) :535-543
[44]   GWAS Reveal Novel Sex-Related Markers and Candidate Genes in Sea Urchin Mesocentrotus nudus [J].
Wang, Quanchao ;
Liu, Ying ;
Wang, Yanxia ;
Jiang, Shaoyu ;
Zhang, Chuanxin ;
Li, Baoquan .
MARINE BIOTECHNOLOGY, 2022, 24 (01) :32-39
[45]   Identification of sex-specific splicing via comparative transcriptome analysis of gonads from sea cucumber Apostichopus japonicus [J].
Wang, Yixin ;
Yang, Yujia ;
Chen, Muyan .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS, 2023, 45
[46]   Identification of sex determination locus in sea cucumber Apostichopus japonicus using genome-wide association study [J].
Wang, Yixin ;
Yang, Yujia ;
Li, Yulong ;
Chen, Muyan .
BMC GENOMICS, 2022, 23 (01)
[47]  
WEIDMAN PJ, 1987, J BIOL CHEM, V262, P15076
[48]   A Homolog of the Cell Apoptosis Susceptibility Gene Involved in Ovary Development of Chinese Shrimp Fenneropenaeus chinensis [J].
Wen, Rong ;
Li, Fuhua ;
Xie, Yusu ;
Li, Shihao ;
Xiang, Jianhai .
BIOLOGY OF REPRODUCTION, 2012, 86 (01)
[49]   Selection shapes turnover and magnitude of sex-biased expression in Drosophila gonads [J].
Whittle, Carrie A. ;
Extavour, Cassandra G. .
BMC EVOLUTIONARY BIOLOGY, 2019, 19 (1)
[50]  
[吴菲菲 Wu Feifei], 2021, [渔业科学进展, Progress in Fishery Sciences], V42, P55
123456