Proteome analysis provides insights into sex differences in Holothuria Scabra

被引：0
作者：
Cheng, Chuhang [1 ,2 ]
Wu, Feifei [3 ]
Xu, Yizhi [4 ]
Ren, Chunhua [5 ]
Chen, Ting [5 ]
Li, Shella [6 ]
Shen, Peihong [2 ]
Jiang, Fajun [1 ]
机构：
[1] Guangxi Acad Sci, Guangxi Acad Marine Sci, Guangxi Key Lab Marine Environm Sci, Nanning, Peoples R China
[2] Guangxi Univ, Coll Life Sci & Technol, Nanning, Peoples R China
[3] Sun Yat Sen Univ, Sch Marine Sci, Zhuhai, Peoples R China
[4] Univ Edinburgh, Sch Biol Sci, Edinburgh, Scotland
[5] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou, Peoples R China
[6] BASIS Int Sch, Guangzhou, Peoples R China
来源：
PLOS ONE | 2024年 / 19卷 / 08期
基金：
中国博士后科学基金;
关键词：
EXPRESSION; FERTILIZATION; PROTEINS; TESTIS; PROTEOLIAISIN; ANTIGEN; GENES; OVARY; RNA;
D O I：
10.1371/journal.pone.0301884
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Sex-determining mechanism is still ambiguous for sea cucumber Holothuria scabra which only manifests gonochorism in gonad. In this study, proteomic analysis was employed to delineate sex-related proteins and genes in gonads of H. scabra, subsequently validated through Quantitative real-time polymerase chain reaction (qRT-PCR). A total of 5,313 proteins were identified via proteome sequencing. Among these, 817 proteins exhibited expression in both the ovary and testis, with 445 proteins displaying up-regulation and 372 proteins showing down-regulation (ovary vs testis). Furthermore, 136 and 69 proteins were identified as ovary-specific and testis-specific Differentially Abundant Proteins (DAPs), respectively. And 9 DAP coding genes which play crucial role in ovary and testis were verified by qRT-PCR. Notably, 24 ovary-bias proteins enriched in ribosome pathway strongly indicated the crucial role of ribosome in ovary. This study serves to furnish novel evidence pertaining to sex differences in H. scabra.
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页数：16
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