Evaluation of VITEK MS Version 3.0 MALDI-TOF for the identification of anaerobes, mycobacteria, Nocardia, and moulds

被引：4

作者：

Sharma, Kusum ^{[1
]}

Angrup, Archana ^{[1
]}

Ghosh, Anup ^{[1
]}

Singh, Shreya ^{[2
]}

Sood, Anshul ^{[1
]}

Arora, Amit ^{[1
]}

Sharma, Megha ^{[3
]}

Sethi, Sunil ^{[1
]}

Rudramurthy, Shivaprakash M. ^{[1
]}

Kaur, Harsimran ^{[1
]}

Ray, Pallab ^{[1
]}

Chakrabarti, Arunaloke ^{[4
]}

机构：

[1] Postgrad Inst Med Educ & Res, Dept Med Microbiol, Chandigarh 160012, India

[2] Dr B R Ambedkar Inst Med Sci AIMS Mohali, Chandigarh 160055, India

[3] All India Inst Med Sci, Dept Med Microbiol, Bilaspur 174001, Himachal Prades, India

[4] Doodhadhari Burfani Hosp & Res Inst, Haridwar 249411, Uttarakhand, India

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2024年 / 110卷 / 04期

关键词：

Anaerobes; Mycobacterium; Nocardia; Moulds; MALDI-TOF MS; VITEK MS; DESORPTION IONIZATION-TIME; ROUTINE IDENTIFICATION; MASS-SPECTROMETRY; SPECIES IDENTIFICATION; MICROFLEX LT; BACTERIA; DIFFERENTIATION; DERMATOPHYTES; PROTEOMICS;

D O I：

10.1016/j.diagmicrobio.2024.116477

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Purpose: The identification of anaerobes, Mycobacterium and Nocardia species, and moulds by MALDI-TOF-MS remains a challenge. This study aimed to evaluate the performance of MALDI-TOF in the identification of these organisms. Methods: A total of 382 strains, comprising 128 (33.5 %) anaerobes, 126(33.0 %) mycobacterial, 113(29.6 %), mycelial fungi, and 15(3.9 %) Nocardia species were evaluated by VITEK MS Version 3.0. The results were compared with the identification of the isolates by DNA sequence analysis. The DNA sequences used for analysis were the 16S rRNA for anaerobic bacteria, hsp65 gene for mycobacteria, whereas both 16S rRNA and hsp65 gene for Nocardia species, and internal transcribed spacer (ITS) and 28S rRNA gene's D1/D2 regions of fungi. Results: The VITEK-MS accurately identified 78.3 % (299/382) of the strains at the species, and 9.4 % (36/382) at the genus level. Misidentifications were observed in 3.9 % (15/382) isolates. Of isolates tested, 8.4 % (32/382) were not identified by the system, and 7.06 % (27/382) were not included in the IVD database. Conclusion: An upgraded VITEK MS V3.0 database provides reasonably accurate and rapid identification of clinically relevant anaerobes, mycobacteria, Nocardia species, and moulds to the species level.

引用

页数：8

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