Multifunctional methyl orange-delaminated Ti3C2 MXene for non-enzymatic/metal-free electrochemical detection of hydrogen peroxide and hydrazine

Developing a biosensor with the ability to conduct non-enzymatic/metal-free multifunctional sensing is challenging and desirable for various fields. This enhances effectiveness and makes it an ideal candidate for measuring biomolecules on the same electrode in a cost-effective, rapid, simple, and easy-to-interpret manner, particularly in point-of-care devices. In this study, methyl orange (MO)-delaminated Ti3C2 MXene was successfully synthesized in a simple one-pot/step manner and utilized as a versatile sensing platform for H2O2 electroreduction and N2H4 electrooxidation. The proposed intriguing technique led to the formation of a greater porosity with a hollow architecture, providing abundant electrocatalytic sites and rapid charge electron transfer rates. The incorporation of MO into the Ti3C2 MXene nanosheets facilitated electronic conductivity and enhanced the effective surface area accessible to the electroactive species with structural robustness. As a result, this approach yielded a wide linear range (0.33 to 1200 and 0.1 to 1350 mu M), a low detection limit (0.05 mu M and 0.01 mu M), and sound recovery in serum for H2O2 and N2H4, along with good selectivity, excellent reproducibility, and remarkable long-term stability.