Antimicrobial activity of apricot kernel extract loaded carboxymethyl chitosan nanoparticles against multidrug resistant wound-skin infection bacteria

In recent years, skin and soft-tissue infections, particularly due to multidrug resistance bacteria (MDR) are generating a serious health crisis to human health. Thus, the current investigation tried to find new promising alternatives such as herbal therapy and biopolymer nanotechnology to combat MDR microbes. Apricot kernels extract was prepared and its amygdalin content was determined by HPLC analysis. Carboxymethyl chitosan nanoparticles (CMC NPs) encapsulated with amygdalin extract (Am ext) were synthesized and characterized through their morphology, particle size, zeta potential and thermal analysis. The antibacterial activity of Am ext, CMC NPs and CMC-Am ext NPs were evaluated against MDR bacteria. Moreover, to confirm the antibacterial action of the samples, bacterial DNA fragmentation analysis was performed. Furthermore, the cyanide ions released from bacterial breakdown of amygdalin was confirmed using Nanocolor Cyanide 08 Test 0-31 kits. The HPLC analysis indicated that amygdalin extracted efficiently from the apricot kernels. The CMC-Am ext NPs exhibited spherical shaped and mono dispersed particles of size 28 nm; physical stability and thermal compatibility. Additionally, CMC-Am ext NPs have significant antibacterial action on all MDR microbes in synergy with Am ext. Moreover, the results confirmed that the cyanide ions were released from amygdalin breakdown by the action of bacteria. Furthermore, the DNA fragmentation analysis confirmed that both Am ext and its nano-encapsulated form caused bacterial cell death by inducing DNA damage. Therefore, these findings demonstrate CMC-Am ext NPs as a novel potential therapeutic agent which can be used as an alternative to the current antibiotics against MDR bacteria.