Gold brocade coated CoFe PBA with enhanced peroxidase-like activity for a chemiluminescent imaging immunoassay

Traditional chemiluminescence (CL) imaging immunoassays usually rely on natural enzymes as catalytic probes, which has hampered their extensive application due to the susceptibility to inactivation of natural enzymes. In response, a gold brocade coated CoFe Prussian blue analogue (CoFe PBA@Au brocade) with enhanced peroxidase-like activity was synthesized and utilized as a powerful label probe for constructing a highly sensitive CL imaging immunosensor targeting disease biomarkers with excellent performance. This research offers a universal strategy for enhancing the sensitivity of CL imaging immunoassays and further expands the application of PBA nanozymes. The prepared gold brocade coated CoFe Prussian blue analogue (CoFe PBA@Au brocade) with enhanced POD-like activity was utilized as a probe for constructing a highly sensitive CL imaging immunosensor targeting biomarkers.