Molecular detection and genotyping of Toxoplasma gondii in stray cat feces from Khorramabad, West Iran

被引：1

作者：

Azizi, Hakim ^{[1
,2
]}

Hataminejad, Maryam ^{[3
,4
,5
]}

Taghipour, Ali ^{[6
]}

Norouzi, Maryam ^{[7
]}

Mirzapour, Aliyar ^{[8
]}

机构：

[1] Zabol Univ Med Sci, Dept Med Parasitol, Zabol, Iran

[2] Zabol Univ Med Sci, Zabol Med Plants Res Ctr, Zabol, Iran

[3] Mazandaran Univ Med Sci, Sch Med, Dept Med Parasitol, Sari, Iran

[4] Mazandaran Univ Med Sci, Toxoplasmosis Res Ctr, Sch Med, Sari, Iran

[5] Mazandaran Univ Med Sci, Student Res Comm, Sari, Iran

[6] Jahrom Univ Med Sci, Zoonoses Res Ctr, Jahrom, Iran

[7] Shahid Beheshti Univ Med Sci, Sch Med, Dept Med Parasitol & Mycol, Tehran, Iran

[8] Islamic Azad Univ, Innovat Med Res Ctr, Sch Med, Dept Med Parasitol & Mycol,Mashhad Branch, Mashhad, Iran

来源：

VETERINARY AND ANIMAL SCIENCE | 2024年 / 25卷

关键词：

Toxoplasma gondii; Prevalence; Stray cats; PCR-RFLP; Genotyping; FERAL CATS; LIFE-CYCLE; PREVALENCE; ANIMALS; AMERICA; REVEALS;

D O I：

10.1016/j.vas.2024.100389

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Cats, being the definitive host of Toxoplasma gondii, have a significant impact on the spread and outbreaks of the parasite. An essential factor in comprehending the transmission pattern of this parasite is an analysis of the genetic diversity distribution in cats infected with T. gondii. This study was aimed at determining the prevalence rate and genotyping of T. gondii in stray cat feces from Khorramabad, West Iran. In the years 2016-2017, 200 cats were sampled to get fresh feces specimens. Parasitological methods were utilized for the identification of oocysts. The DNA was isolated from the feces using a commercially available Genomic Mini Kit. In order to identify the genetic composition of T. gondii, we employed PCR-RFLP, sequencing, and phylogenetic analysis of the GRA6 target gene. No one of the samples tested positive for parasitology techniques. A total of 6.5 % (13/200) samples were positive when using the GRA6-PCR method. Based on PCR-RFLP results, all 13 samples were of T. gondii type III genotype. The nucleotide sequences of two samples from this study were found to be 5 % different from those of 12 references of T. gondii and one strain of Hammondia hamondi that was used as an external control. Based on the findings, molecular tests are more sensitive than parasitological methods. The RFLP approach revealed that type III of T. gondii is the prevailing and important genotype in Khorramabad, West Iran.

引用

页数：5