Optimization and Stability Assessment of Monochamus alternatus Antimicrobial Peptide MaltAtt-1 in Komagataella phaffii GS115 for the Control of Pine Wood Nematode

被引：0

作者：

Jiang, Di ^{[1
,2
]}

Xu, Xuhuizi ^{[1
,2
]}

Wang, Zeguang ^{[1
,2
]}

Yu, Chao ^{[1
]}

Wang, Zeqing ^{[1
]}

Xu, Yuda ^{[1
,2
]}

Chu, Xu ^{[1
,2
]}

Li, Ming ^{[1
]}

Zhang, Feiping ^{[1
,2
]}

Hu, Xia ^{[1
,2
]}

机构：

[1] Fujian Agr & Forestry Univ, Forestry Coll, Fuzhou 350002, Peoples R China

[2] Fujian Agr & Forestry Univ, Key Lab Integrated Pest Management Ecol Forests, Fuzhou 350002, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 16期

基金：

中国国家自然科学基金;

关键词：

antimicrobial peptide; induction optimization; Komagataella phaffii; stability; PICHIA-PASTORIS; EXPRESSION;

D O I：

10.3390/ijms25168555

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MaltAtt-1 is an antimicrobial peptide isolated from Monochamus alternatus with nematocidal activity against pine wood nematode. In this study, a eukaryotic expression system based on Komagataella phaffii GS115 was established, and its secretory expression of MaltAtt-1 was realized. The basic properties and secondary and tertiary structures of the antimicrobial peptide MaltAtt-1 were identified by bioinformatics analysis. MaltAtt-1 is a hydrophilic stable protein, mainly composed of an alpha-helix (Hh), beta-folds (Ee), and irregular curls (Cc). The optimal fermentation conditions for MaltAtt-1 were determined by a single-factor test and the Box-Behnken response surface method, including an induction time of 72 h, induction temperature of 30 degrees C, culture medium of pH 7.6, methanol volume fraction of 2.0%, and an initial glycerol concentration of 1%. The stability of MaltAtt-1 indicated its resistant to UV irradiation and repeated freezing and thawing, but the antibacterial activity decreased significantly under the influence of high temperature and a strong acid and base, and it decreased significantly to 1.1 cm and 0.83 cm at pH 2.0 and pH 10.0, respectively. The corrected mortality of B. xylophilus achieved 71.94% in 3 h at a concentration of 300 mg.L-1 MaltAtt-1 exposure. The results provide a theoretical basis for the antimicrobial peptide MaltAtt-1 to become a new green and efficient nematicide.

引用

页数：13