Laser Fragmentation of Green Tea-synthesized Silver Nanoparticles and Their Blood Toxicity: Effect of Laser Wavelength on Particle Diameters

Background: The efficacy of fractionation is significantly impacted by the colloidal particles' spontaneous absorption of laser beam radiation. The classification of silver nanoparticles during fragmentation processing is regulated through the collection of a combination of laser pulses with wavelengths of 1064 nm and 532 nm.Aims and Objectives: This study presents an investigation of the efficacy of a plant extract in conjunction with the incorporation of supplementary silver nanoparticles, as well as the generation of smaller-sized silver nanoparticles using laser fragmentation.and then measure thier toxity on the blood.Results: Ag nanoparticles were synthesized using pulsed laser fragmentation on green tea AgNPs. The synthesis process involved the utilization of a Q-switch Nd:YAG laser with wavelengths of 1064 nm and 532 nm, with energy ranging from 200 to 1000 mJ. Initially, a silver nano colloid was synthesized through the process of fragmented of the Ag target using the second harmonic generation of 532 nm at various energy levels. The optimal energy within the selected wavelengths was determined in order to facilitate the ultimate comparison. Transmission electron microscopy (TEM) was used to determine surface morphology and average particle size, while a spectrophotometer was used to analyses UV light's spectrum characteristics. The measurements focused on the surface plasmon resonance (SPR) phenomenon. The absorption spectra of silver nanoparticles exhibit distinct and prominent peaks at wavelengths of 405 nm and 415 nm. The mean diameter of the silver nanoparticles was found to be 16 nm and 20 nm, corresponding to wavelengths of 1064 nm and 532 nm, respectively.Conclusion: As a consequence, there is a decrease in the range of particle sizes and a decrease in the mean size to lower magnitudes, resulting in a very stable colloid. This particular methodology has demonstrated considerable efficacy in the production of colloidal suspensions with the intended particle dimensions. Moreover, by the analysis of nanoparticles in human blood, no discernible alterations in the blood constituents were seen, indicating their non-toxic nature.