Impact of Hepatitis Delta Virus Infection on the Selection of Hepatitis B Surface Antigen Mutations

被引：1

作者：

Baruti, Kabo ^{[1
,2
]}

Choga, Wonderful T. ^{[1
,3
]}

Phinius, Bonolo B. ^{[1
,3
]}

Phakedi, Basetsana ^{[1
]}

Bhebhe, Lynnette ^{[1
]}

Mpebe, Gorata G. A. ^{[1
]}

Motshosi, Patience C. ^{[1
]}

Ratsoma, Tsholofelo ^{[1
]}

Moyo, Sikhulile ^{[1
,4
,5
]}

Jongman, Mosimanegape ^{[1
,2
]}

Anderson, Motswedi ^{[1
,6
,7
]}

Gaseitsiwe, Simani ^{[1
,4
]}

机构：

[1] Botswana Harvard Hlth Partnership, Res Lab, Gaborone Private Bag BO 320, Gaborone, Botswana

[2] Univ Botswana, Fac Sci, Dept Biol Sci, Gaborone Private Bag 00704, Gaborone, Botswana

[3] Univ Botswana, Fac Hlth Sci, Sch Allied Hlth Profess, Gaborone Private Bag 00704, Gaborone, Botswana

[4] Harvard TH Chan Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA

[5] Stellenbosch Univ, Dept Pathol, Div Med Virol, ZA-7500 Cape Town, South Africa

[6] Afr Hlth Res Inst AHRI, Durban, South Africa

[7] Francis Crick Inst, London NW1 2BE, England

来源：

GENES | 2024年 / 15卷 / 08期

基金：

比尔及梅琳达.盖茨基金会; 美国国家卫生研究院; 英国惠康基金;

关键词：

mutations; hepatitis delta virus; hepatitis B virus; people living with HIV; Botswana; HBV; PREVALENCE;

D O I：

10.3390/genes15080982

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The interaction of multiple viruses in one host is thought to enhance the development of mutations. However, the impact of hepatitis D virus (HDV) positivity on the development of unique hepatitis B virus (HBV) mutations among people living with human immunodeficiency virus (HIV) (PLWH) remains poorly understood in African countries, including Botswana. We used HBV sequences generated from the Botswana Combination Prevention Project (BCPP), which is the largest pair-matched cluster-randomized HIV trial in Botswana. Only participants with available HBV sequences (n = 55) were included in our study ([HIV/HBV-positive (n = 50) and HIV/HBV/HDV-positive (n = 5)]. Geno2pheno was used to determine HBV genotypes, and HBV surface region sequences (all subgenotype A1) were aligned in AliView for mutational analysis, while the impact of mutations was assessed using Phyre2. Our results identified 182 common mutations between the two groups. In the HIV/HBV/HDV cohort, only three mutations (L95W, W156Q, C221Y) were classified as deleterious, with only L95W being the most frequent. In the HIV/HBV cohort, four mutations (W199R, C221A, C221S, W223G) were also classified as deleterious. Our results demonstrate the presence of unique HBV mutations among the HIV/HBV/HDV-positive cohort. Functional characterization of these mutations is recommended to determine their effect on HDV.

引用

页数：10

共 37 条

[1] Entry of hepatitis delta virus requires the conserved cysteine residues of the hepatitis B virus envelope protein antigenic loop and is blocked by inhibitors of thiol-disulfide exchange [J].