Assessment of Tumor Cell Invasion and Radiotherapy Response in Experimental Glioma by Magnetic Resonance Elastography

被引:2
|
作者
Fels-Palesandro, Hannah [1 ,2 ]
Heuer, Sophie [3 ,4 ,5 ]
Boztepe, Berin [1 ,6 ]
Streibel, Yannik [1 ]
Ungermann, Johannes [1 ]
Pan, Chenchen [3 ,4 ,5 ]
Scheck, Jonas G. [1 ,2 ]
Fischer, Manuel [1 ]
Sturm, Volker J. [1 ]
Azorin, Daniel D. [3 ,4 ,5 ,7 ]
Karimian-Jazi, Kianush [1 ]
Annio, Giacomo [8 ,9 ]
Abdollahi, Amir [2 ]
Weidenfeld, Ina [1 ,2 ]
Wick, Wolfgang [3 ,4 ,5 ]
Venkataramani, Varun [3 ,4 ,5 ]
Heiland, Sabine [1 ]
Winkler, Frank [3 ,4 ,5 ]
Bendszus, Martin [1 ]
Sinkus, Ralph [8 ,9 ]
Breckwoldt, Michael O. [1 ,6 ]
Schregel, Katharina [1 ]
机构
[1] Heidelberg Univ Hosp, Dept Neuroradiol, Heidelberg, Germany
[2] German Canc Res Ctr, Clin Cooperat Unit Translat Radiat Oncol, Heidelberg, Germany
[3] Heidelberg Univ Hosp, Dept Neurol, Heidelberg, Germany
[4] Heidelberg Univ Hosp, Natl Ctr Tumor Dis NCT, Heidelberg, Germany
[5] German Canc Res Ctr, Clin Cooperat Unit Neurooncol, Heidelberg, Germany
[6] German Canc Res Ctr, Clin Cooperat Unit Neuroimmunol & Brain Tumor Immu, Heidelberg, Germany
[7] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, Basel, Switzerland
[8] Univ Paris, INSERM UMRS1148, Lab Vasc Translat Sci, Paris, France
[9] Kings Coll London, Sch Biomed Engn & Imaging Sci, London, England
关键词
brain tumor; glioma invasion; MR elastography; tissue biomechanics; white matter; stiffness; IN-VIVO; PLATFORM; TISSUE;
D O I
10.1002/jmri.29567
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Background Gliomas are highly invasive brain neoplasms. MRI is the most important tool to diagnose and monitor glioma but has shortcomings. In particular, the assessment of tumor cell invasion is insufficient. This is a clinical dilemma, as recurrence can arise from MRI-occult glioma cell invasion. Hypothesis Tumor cell invasion, tumor growth and radiotherapy alter the brain parenchymal microstructure and thus are assessable by diffusion tensor imaging (DTI) and MR elastography (MRE). Study Type Experimental, animal model. Animal Model Twenty-three male NMRI nude mice orthotopically implanted with S24 patient-derived glioma cells (experimental mice) and 9 NMRI nude mice stereotactically injected with 1 mu L PBS (sham-injected mice). Field Strength/Sequence2D and 3D T2-weighted rapid acquisition with refocused echoes (RARE), 2D echo planar imaging (EPI) DTI, 2D multi-slice multi-echo (MSME) T2 relaxometry, 3D MSME MRE at 900 Hz acquired at 9.4 T (675 mT/m gradient strength). Assessment Longitudinal 4-weekly imaging was performed for up to 4 months. Tumor volume was assessed in experimental mice (n = 10 treatment-control, n = 13 radiotherapy). The radiotherapy subgroup and 5 sham-injected mice underwent irradiation (3 x 6 Gy) 9 weeks post-implantation/sham injection. MRI-/MRE-parameters were assessed in the corpus callosum and tumor core/injection tract. Imaging data were correlated to light sheet microscopy (LSM) and histology. Statistical Tests Paired and unpaired t-tests, a P-value <= 0.05 was considered significant. Results From week 4 to 8, a significant callosal stiffening (4.44 +/- 0.22 vs. 5.31 +/- 0.29 kPa) was detected correlating with LSM-proven tumor cell invasion. This was occult to all other imaging metrics. Histologically proven tissue destruction in the tumor core led to an increased T2 relaxation time (41.65 +/- 0.34 vs. 44.83 +/- 0.66 msec) and ADC (610.2 +/- 12.27 vs. 711.2 +/- 13.42 x 10-6 mm2/s) and a softening (5.51 +/- 0.30 vs. 4.24 +/- 0.29 kPa) from week 8 to 12. Radiotherapy slowed tumor progression. Data Conclusion MRE is promising for the assessment of key glioma characteristics. Evidence LevelNA Technical EfficacyStage 2
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收藏
页码:1203 / 1218
页数:16
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