Promising approach for targeting ROBO1 with CAR NK cells to combat ovarian cancer primary tumor cells and organoids

被引：0

作者：

Zhu, Yan ^{[1
]}

Wang, Luanhong ^{[1
]}

Jiang, Biyu ^{[2
]}

Wang, Yini ^{[1
]}

Wu, Qing ^{[1
]}

Hong, Sihua ^{[2
]}

Wang, Xiaojing ^{[1
]}

Li, Yuancheng ^{[1
]}

Guan, Tian ^{[2
]}

Zeng, Haoyu ^{[2
]}

Li, Congzhu ^{[1
]}

机构：

[1] Shantou Univ, Canc Hosp, Med Coll, Dept Gynecol Oncol, Shantou, Guangdong, Peoples R China

[2] Guangdong Procapzoom Biosci Co, Dept Canc Res, Guangzhou, Guangdong, Peoples R China

来源：

FUTURE SCIENCE OA | 2024年 / 10卷 / 01期

关键词：

CAR-NK; organoid; ovarian cancer; PBMCs; ROBO1; NATURAL-KILLER-CELLS; EXPRESSION; PATTERNS;

D O I：

10.2144/fsoa-2023-0135

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aim: This study aimed to explore using peripheral blood mononuclear cell (PBMC)-derived chimeric antigen receptor (CAR) NK cells targeting ROBO1 as a personalized medicine approach for ovarian cancer. Methods: A two-step strategy generated ROBO1-targeted CAR NK cells from PBMCs of ovarian cancer patients. Efficacy was evaluated using xCELLigence RTCA, CCK-8 and Live/Dead fluorescence assays. Results: ROBO1-NK cells exhibited higher efficiency in eradicating primary ovarian cancer cells and lysing ovarian tumor organoids compared with primary NK cells without ROBO1-CAR modification. Conclusion: These findings highlight the potential of developing ROBO1-targeted CAR-NK cells from patients' PBMCs as a personalized treatment option for ovarian cancer.

引用

页数：12

共 32 条

[1] A Roundabout Way to Cancer
Ballard, Mimmi S.
Hinck, Lindsay
[J]. GUIDANCE MOLECULES IN CANCER AND TUMOR ANGIOGENESIS, 2012, 114 : 187 - 235
[2] Selection and expansion of natural killer cells for NK cell-based immunotherapy
Becker, Petra S. A.
Suck, Garnet
Nowakowska, Paulina
Ullrich, Evelyn
Seifried, Erhard
Bader, Peter
Tonn, Torsten
Seidl, Christian
[J]. CANCER IMMUNOLOGY IMMUNOTHERAPY, 2016, 65 (04) : 477 - 484
[3] IL-2 Stimulated but Not Unstimulated NK Cells Induce Selective Disappearance of Peripheral Blood Cells: Concomitant Results to a Phase I/II Study
Brehm, Claudia
Huenecke, Sabine
Quaiser, Andrea
Esser, Ruth
Bremm, Melanie
Kloess, Stephan
Soerensen, Jan
Kreyenberg, Hermann
Seidl, Christian
Becker, Petra S. A.
Muehl, Heiko
Klingebiel, Thomas
Bader, Peter
Passweg, Jakob R.
Schwabe, Dirk
Koehl, Ulrike
[J]. PLOS ONE, 2011, 6 (11):
[4] Escape of tumor cells from the NK cell cytotoxic activity
Cantoni, Claudia
Wurzer, Hannah
Thomas, Clement
Vitale, Massimo
[J]. JOURNAL OF LEUKOCYTE BIOLOGY, 2020, 108 (04) : 1339 - 1360
[5] Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer
Chen, Minhua
Li, Yutong
Wu, Yu
Xie, Siqi
Ma, Jie
Yue, Jingjing
Lv, Rong
Tian, Zhigang
Fang, Fang
Xiao, Weihua
[J]. CANCERS, 2021, 13 (22)
[6] Insight into mechanisms associated with cytokine release syndrome and neurotoxicity after CD19 CAR-T cell immunotherapy
Chou, Cassie K.
Turtle, Cameron J.
[J]. BONE MARROW TRANSPLANTATION, 2019, 54 (Suppl 2) : 780 - 784
[7] Glucocorticoid Regulation of SLIT/ROBO Tumour Suppressor Genes in the Ovarian Surface Epithelium and Ovarian Cancer Cells
Dickinson, Rachel E.
Fegan, K. Scott
Ren, Xia
Hillier, Stephen G.
Duncan, W. Colin
[J]. PLOS ONE, 2011, 6 (11):
[8] Distinct but overlapping expression patterns of two vertebrate slit homologs implies functional roles in CNS development and organogenesis
Holmes, GP
Negus, K
Burridge, L
Raman, S
Algar, E
Yamada, T
Little, MH
[J]. MECHANISMS OF DEVELOPMENT, 1998, 79 (1-2) : 57 - 72
[9] Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
Ingegnere, Tiziano
Mariotti, Francesca Romana
Pelosi, Andrea
Quintarelli, Concetta
De Angelis, Biagio
Tumino, Nicola
Besi, Francesca
Cantoni, Claudia
Locatelli, Franco
Vacca, Paola
Moretta, Lorenzo
[J]. FRONTIERS IN IMMUNOLOGY, 2019, 10
[10] Ovarian cancer
Jayson, Gordon C.
Kohn, Elise C.
Kitchener, Henry C.
Ledermann, Jonathan A.
[J]. LANCET, 2014, 384 (9951) : 1376 - 1388

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