CO2-based production of C2-C6 acids and alcohols: The potential of novel Clostridia

被引:6
作者
Thunuguntla, Rahul [1 ]
Atiyeh, Hasan K. [1 ,3 ]
Huhnke, Raymond L. [1 ]
Tanner, Ralph S. [2 ]
机构
[1] Oklahoma State Univ, Biosyst & Agr Engn, Stillwater, OK 74078 USA
[2] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK USA
[3] Oklahoma State Univ, Dept Biosyst & Agr Engn, 214 Ag Hall, Stillwater, OK 74078 USA
基金
美国食品与农业研究所;
关键词
Novel acetogens; Ethanol; Butanol; Hexanol; CO2; conversion; COST FERMENTATION MEDIUM; ETHANOL-PRODUCTION; SYNGAS FERMENTATION; CARBON-MONOXIDE; CONVERSION; CARBOXIDIVORANS; BUTANOL; DIOXIDE;
D O I
10.1016/j.biteb.2023.101713
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study compared conversion of CO2 into C2-C6 products by Clostridium carboxidivorans P7 and P20, C. ragsdalei P11, C. ljungdahlii P14 and C. muellerianum P21. The effects of medium components and inoculum growth conditions on CO2 fermentation were evaluated. Fermentations were performed in 250-mL bottle assays at 125 rpm and 37 degrees C, using H2:CO2:N2 (60:20:20) that was fed daily for 360 h. Results showed that CO2 fermentations in corn steep liquor medium outperformed those in yeast extract-based media, with strain P11 producing 4.5 g/L ethanol and strain P21 producing 0.5 g/L hexanol. Alcohol formation was 2- to 4-fold higher using inocula pre-grown on syngas compared to H2:CO2. Strain P21 demonstrated higher C4 and C6 alcohols production compared to other strains. Further improvements are necessary to enhance activity and selectivity for converting CO2 into desired products.
引用
收藏
页数:11
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