CO2-based production of C2-C6 acids and alcohols: The potential of novel Clostridia

被引：6

作者：

Thunuguntla, Rahul ^{[1
]}

Atiyeh, Hasan K. ^{[1
,3
]}

Huhnke, Raymond L. ^{[1
]}

Tanner, Ralph S. ^{[2
]}

机构：

[1] Oklahoma State Univ, Biosyst & Agr Engn, Stillwater, OK 74078 USA

[2] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK USA

[3] Oklahoma State Univ, Dept Biosyst & Agr Engn, 214 Ag Hall, Stillwater, OK 74078 USA

来源：

BIORESOURCE TECHNOLOGY REPORTS | 2024年 / 25卷

基金：

美国食品与农业研究所;

关键词：

Novel acetogens; Ethanol; Butanol; Hexanol; CO2; conversion; COST FERMENTATION MEDIUM; ETHANOL-PRODUCTION; SYNGAS FERMENTATION; CARBON-MONOXIDE; CONVERSION; CARBOXIDIVORANS; BUTANOL; DIOXIDE;

D O I：

10.1016/j.biteb.2023.101713

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study compared conversion of CO2 into C2-C6 products by Clostridium carboxidivorans P7 and P20, C. ragsdalei P11, C. ljungdahlii P14 and C. muellerianum P21. The effects of medium components and inoculum growth conditions on CO2 fermentation were evaluated. Fermentations were performed in 250-mL bottle assays at 125 rpm and 37 degrees C, using H2:CO2:N2 (60:20:20) that was fed daily for 360 h. Results showed that CO2 fermentations in corn steep liquor medium outperformed those in yeast extract-based media, with strain P11 producing 4.5 g/L ethanol and strain P21 producing 0.5 g/L hexanol. Alcohol formation was 2- to 4-fold higher using inocula pre-grown on syngas compared to H2:CO2. Strain P21 demonstrated higher C4 and C6 alcohols production compared to other strains. Further improvements are necessary to enhance activity and selectivity for converting CO2 into desired products.

引用

页数：11

共 47 条

[1] CO2 utilization: an enabling element to move to a resource- and energy-efficient chemical and fuel production [J].

Ampelli, Claudio ;

Perathoner, Siglinda ;

Centi, Gabriele .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 2015, 373 (2037)

[2] Expanding the product portfolio of carbon dioxide and hydrogen-based gas fermentation with an evolved strain of Clostridium carboxidivorans [J].

Antonicelli, G. ;

Ricci, L. ;

Tarraran, L. ;

Garofalo, S. Fraterrigo ;

Re, A. ;

Vasile, N. S. ;

Verga, F. ;

Pirri, C. F. ;

Menin, B. ;

Agostino, V. .

BIORESOURCE TECHNOLOGY, 2023, 387

[3] Sustainable Conversion of Carbon Dioxide: An Integrated Review of Catalysis and Life Cycle Assessment [J].

Artz, Jens ;

Mueller, Thomas E. ;

Thenert, Katharina ;

Kleinekorte, Johanna ;

Meys, Raoul ;

Sternberg, Andre ;

Bardow, Andre ;

Leitner, Walter .

CHEMICAL REVIEWS, 2018, 118 (02) :434-504

[4]

Atiyeh H.K., 2021, U.S. Patent, Patent No. [11,180,779B2, 11180779, 11,180,779]

[5] Selecting emerging CO2 utilization products for short- to mid-term deployment [J].

Chauvy, Remi ;

Meunier, Nicolas ;

Thomas, Diane ;

De Weireld, Guy .

APPLIED ENERGY, 2019, 236 :662-680

[6] A low-cost fermentation medium for thermophilic protease production by Streptomyces sp 594 using feather meal and corn steep liquor [J].

De Azeredo, L. A. I. ;

De Lima, M. B. ;

Coelho, R. R. R. ;

Freire, D. M. G. .

CURRENT MICROBIOLOGY, 2006, 53 (04) :335-339

[7] Ethanol production during semi-continuous syngas fermentation in a trickle bed reactor using Clostridium ragsdalei [J].

Devarapalli, Mamatha ;

Atiyeh, Hasan K. ;

Phillips, John R. ;

Lewis, Randy S. ;

Huhnke, Raymond L. .

BIORESOURCE TECHNOLOGY, 2016, 209 :56-65

[8] Clostridium muellerianum sp. nov., a carbon monoxide-oxidizing acetogen isolated from old hay [J].

Doyle, D. Annie ;

Smith, Parker R. ;

Lawson, Paul A. ;

Tanner, Ralph S. .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2022, 72 (03)

[9]

EIA, 2022, about us

[10] Functional Expression of the Clostridium ljungdahlii Acetyl-Coenzyme A Synthase in Clostridium acetobutylicum as Demonstrated by a Novel In Vivo CO Exchange Activity En Route to Heterologous Installation of a Functional Wood-Ljungdahl Pathway [J].

Fast, Alan G. ;

Papoutsakis, Eleftherios T. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2018, 84 (07)

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