Analysis of volatile floral fragrance components of Camellia yuhsienensis Hu by HS-SPME/GC-MS

The flowers of selected Camellia yuhsienensis Hu specimens were studied at different stages of flowering to determine the volatile components of floral fragrance and how their concentrations changed during efflorescence. The mechanism of fragrance formation and release was also studied in order to provide a theoretical basis for the practical use of C. yuhsienensis Hu flowers. Headspace solid-phase microextraction (HS-SPME) was used to extract the natural floral volatile oils of C. yuhsienensis Hu at the different stages, and the floral fragrance components were analysed by gas chromatography-mass spectrometry (GC-MS). A total of 31, 59 and 55 compounds were detected at the budding, early-blooming and full-blooming stages respectively. These floral fragrance components included terpene alcohols, as well as other aromatic and aliphatic compounds, and the relative concentrations of the various compounds at different stages of flowering, were different, and those of fatty alcohols and terpene alcohols having higher relative contents at all stages. The main component at the budding stage of C. yuhsienensis Hu was ocimene, which has a grassy fragrance, accompanied by the scent of neroli oil, which gives it a refreshing, gentle and sweet, and explains why the floral fragrance of C. yuhsienensis Hu at the budding stage is extremely thin: the main component during the early-blooming stage was 3-carene. A higher percentage of 3-carene increased the sweetness of the scent, manifesting the typical fragrant characteristics of C. yuhsienensis Hu. The main identified compound at the full-blooming stage was alpha-farnesene, which has a delicate fragrance, accompanied by a balsamic aroma, similar to the scent of gardenia, which is consistent with the sensory characteristics of C. yuhsienensis Hu.