Chemically Defined Production of Tri-Lineage Human iPSC-Derived Cardiac Spheroids

被引：7

作者：

Arzt, Madelyn ^{[1
,2
,3
,4
]}

Pohlman, Stephany ^{[1
,2
,3
,4
,5
]}

Mozneb, Maedeh ^{[1
,2
,3
,4
]}

Sharma, Arun ^{[1
,2
,3
,4
]}

机构：

[1] Cedars Sinai Med Ctr, Board Governors Regenerat Med Inst, Los Angeles, CA 90048 USA

[2] Cedars Sinai Med Ctr, Smidt Heart Inst, Los Angeles, CA 90048 USA

[3] Cedars Sinai Med Ctr, Dept Biomed Sci, Los Angeles, CA 90048 USA

[4] Cedars Sinai Med Ctr, Canc Inst, Los Angeles, CA 90048 USA

[5] Calif State Univ Channel Isl, CIRM Bridges Stem Cell Res Program, Channel Isl, CA USA

来源：

CURRENT PROTOCOLS | 2023年 / 3卷 / 05期

关键词：

cardiac fibroblasts; cardiac spheroids; cardiomyocytes; endothelial cells; iPSCs; ORGANOIDS; CARDIOMYOCYTES; MICROTISSUES; MATURATION; REVEALS;

D O I：

10.1002/cpz1.767

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cardiac spheroids derived from human induced pluripotent stem cells (hiPSC-cardiac spheroids) represent a powerful three-dimensional (3D) model for examining cardiac physiology and for drug toxicity screening. Recent advances with self-organizing, multicellular cardiac organoids highlight the capability of directed stem cell differentiation approaches to recapitulate the composition of the human heart in vitro. Using hiPSC-derived cardiomyocytes (hiPSC-CMs), hiPSC-derived endothelial cells (hiPSC-ECs), and hiPSC-derived cardiac fibroblasts (hiPSC-CFs) is advantageous for enabling tri-cellular crosstalk within a multilineage system and for generating patient-specific models. Chemically defined medium containing factors needed to simultaneously maintain hiPSC-CMs, hiPSC-ECs, and hiPSC-CFs is used to produce the spheroid system. In this article, we present protocols to illustrate the methods for conducting small-molecule-mediated differentiations of hiPSCs into cardiomyocytes, endothelial cells, and cardiac fibroblasts, as well as to assemble the fully integrated cardiac spheroids. (c) 2023 Wiley Periodicals LLC.

引用

页数：25