Antimicrobial Resistance and Genomic Characterization of Campylobacter jejuni and Campylobacter coli Isolated from Retail Chickens in Beijing, China

被引:3
作者
Bai, Yao [1 ]
Ma, Jiaqi [2 ]
Li, Fengqin [1 ]
Yang, Baowei [2 ]
Ren, Xiu [3 ]
Wang, Yeru [1 ]
Hu, Yujie [1 ]
Dong, Yinping [1 ]
Wang, Wei [1 ]
Zhang, Jing [1 ]
Yan, Shaofei [1 ]
Cui, Shenghui [3 ]
机构
[1] China Natl Ctr Food Safety Risk Assessment, NHC Key Lab Food Safety Risk Assessment, Beijing 100022, Peoples R China
[2] Northwest Agr & Forestry Sci & Technol Univ, Coll Food Sci & Engn, Xianyang 712100, Shaanxi, Peoples R China
[3] Natl Inst Food & Drug Control, Beijing 100050, Peoples R China
基金
中国国家自然科学基金;
关键词
Campylobacter jejuni; Campylobacter coli; antimicrobial resistance; whole genome sequencing;
D O I
10.3390/microorganisms12081601
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Objective Campylobacter species are the main causes of foodborne illness worldwide, posing significant threats to public health. This study aimed to investigate the antibiotic resistance and genomic characterization of C. jejuni/C.coli from retail chickens in Beijing. Methods Antimicrobial susceptibility testing was conducted on 126 C. jejuni/C. coli isolated from retail chickens in Beijing, following CLSI protocols. Whole genomes of all isolates were sequenced using the Illumina platform. Results More C. coli (83.82%) showed multi-drug resistance than C. jejuni (8.62%). Genomic analysis demonstrated 42 sequence types (STs) and 12 clonal complexes (CCs), from which CC828 and CC52 were dominant. cdtA, cdtB and cdtC encoding cytotoxic protein were present spontaneously in most C. jejuni but not found in any C. coli isolates. The abundances of antibiotic resistance genes (ARGs) and virulence genes (VGs) in C. jejuni and C. coli were significantly different, with ARGs numbered in C. coli and VGs in C. jejuni. Conclusions High prevalence of multi-drug resistance C. coli and C. jejuni isolated from Beijing chickens were challenging clinical antibiotic usages in the treatment of Campylobacter infection. The surveillance of particular C. jejuni and C. coli STs correlated with higher resistance and virulence needs to be strengthened in the future.
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页数:13
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