Detection of the epidemic Pseudomonas aeruginosa AUST-03 (ST242) strain in people with cystic fibrosis in South Africa

被引：0

作者：

Hamiwe, Thabo ^{[1
]}

White, Debbie A. ^{[2
]}

Kwenda, Stanford ^{[3
]}

Ismail, Arshad ^{[3
,4
,5
]}

Klugman, Susan ^{[2
]}

Van Bruwaene, Lore ^{[6
,7
]}

Goga, Ameena ^{[6
,8
]}

Kock, Marleen M. ^{[1
,9
]}

Smith, Anthony M. ^{[1
,10
]}

Ehlers, Marthie M. ^{[1
,9
]}

机构：

[1] Univ Pretoria, Fac Hlth Sci, Dept Med Microbiol, Pretoria, South Africa

[2] Univ Witwatersrand, Charlotte Maxeke Johannesburg Acad Hosp, Fac Hlth Sci, Dept Paediat Pulmonol, Johannesburg, South Africa

[3] Natl Inst Communicable Dis, Div Natl Hlth Lab Serv, Sequencing Core Facil, Johannesburg, South Africa

[4] Univ Venda, Dept Biochem & Microbiol, Thohoyandou, South Africa

[5] Durban Univ Technol, Inst Water & Wastewater Technol, Durban, South Africa

[6] Univ Pretoria, Steve Biko Acad Hosp, Dept Paediat Pulmonol, Pretoria, South Africa

[7] St Pierre Hosp, Ctr Hosp Univ, Dept Pediat, Brussels, Belgium

[8] South African Med Res Council, Pretoria, South Africa

[9] Natl Hlth Lab Serv, Tshwane Acad Div, Dept Microbiol, Pretoria, South Africa

[10] Natl Inst Communicable Dis, Ctr Enter Dis, Div Natl Hlth Lab Serv, Johannesburg, South Africa

来源：

PEDIATRIC PULMONOLOGY | 2024年 / 59卷 / 12期

关键词：

antibiotic resistance; cystic fibrosis; Pseudomonas aeruginosa AUST-03; South Africa; TRANSMISSIBLE STRAINS; CLINICAL-OUTCOMES; RESISTANCE; MECHANISMS;

D O I：

10.1002/ppul.27202

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

Introduction: Pseudomonas aeruginosa AUST-03 (ST242) has been reported to cause epidemics in people with CF (pwCF) from Australia and has been associated with multidrug resistance and increased morbidity and mortality. Here, we report an epidemic P. aeruginosa (AUST-03) strain in South African pwCF detected at a public hospital and characterize the genomic antibiotic resistance determinants. Methods: The P. aeruginosa AUST-03 (ST242) study isolates were analysed with whole genome sequencing using the Illumina NextSeq2000 platform. Raw sequencing reads were processed using the Jekesa pipeline and multilocus sequence typing and genomic antibiotic resistance characterization was performed using public databases. Genetic relatedness between the study isolates and global P. aeruginosa ST242 from public databases was determined using a maximum-likelihood phylogenetic tree. Antibiotic susceptibility testing was performed using the disk diffusion and broth microdilution techniques. Results: A total of 11 P. aeruginosa AUST-03 isolates were isolated from two children with CF. The majority (8/11) of these isolates were multidrug-resistant (MDR) or extensively drug resistant; and the multidrug efflux pumps MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY-OprM were the most clinically relevant antibiotic resistance determinants and were detected in all of the isolates. The study isolates were the most closely related to a 2020 P. aeruginosa AUST-03 (ST242) CF isolate from Russia. Conclusion: Epidemic MDR P. aeruginosa strains are present at South African public CF clinics and need to be considered when implementing segregation and infection control strategies to prevent possible spread and outbreaks.

引用

页码：3340 / 3348

页数：9