Platelet Phenotyping by Full Spectrum Flow Cytometry

被引：5
作者：
Spurgeon, Benjamin E. J. [1 ]
Frelinger III, Andrew L. [1 ]
机构：
[1] Harvard Med Sch, Ctr Platelet Res Studies, Dana Farber Boston Childrens Canc & Blood Disorder, Boston, MA 02115 USA
来源：
CURRENT PROTOCOLS | 2023年 / 3卷 / 02期
关键词：
biomarkers; blood platelets; flow cytometry; phenotype; platelet activation; MONOCLONAL-ANTIBODIES; ACTIVATION;
D O I：
10.1002/cpz1.687
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Platelets play key roles in hemostasis, immunity, and inflammation, and tests of platelet phenotype and function are useful in studies of disease biology and pathology. Full spectrum flow cytometry offers distinct advantages over standard tests and enables the sensitive and simultaneous detection of many biomarkers. A typical assay provides a wealth of information on platelet biology and allows the assessment of in vivo activation and in vitro reactivity, as well as the discovery of novel phenotypes. Here, we describe the analysis of platelets by full spectrum flow cytometry and discuss a range of controls and methods for interpreting results. (c) 2023 Wiley Periodicals LLC.Basic Protocol: Platelet phenotyping by full spectrum flow cytometrySupport Protocol 1: Spectral unmixingSupport Protocol 2: Data preprocessing
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页数：13
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